J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aparicio, F.
Right arrow Articles by Bol, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aparicio, F.
Right arrow Articles by Bol, J. F.
Agricola
Right arrow Articles by Aparicio, F.
Right arrow Articles by Bol, J. F.
Journal of General Virology (2001), 82, 947-951.
© 2001 Society for General Microbiology

Plant

Recognition of cis-acting sequences in RNA 3 of Prunus necrotic ringspot virus by the replicase of Alfalfa mosaic virus

F. Aparicio1, J. A. Sánchez-Navarro2, R. C. L. Olsthoorn2, V. Pallás1 and J. F. Bol2

Instituto de Biología Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia, Spain1
Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands2

Author for correspondence: John F. Bol. Fax +31 71 527 4469. e-mail J.BOL{at}chem.LeidenUniv.nl

Alfalfa mosaic virus (AMV) and Prunus necrotic ringspot virus (PNRSV) belong to the genera Alfamovirus and Ilarvirus, respectively, of the family Bromoviridae. Initiation of infection by AMV and PNRSV requires binding of a few molecules of coat protein (CP) to the 3' termini of the inoculum RNAs and the CPs of the two viruses are interchangeable in this early step of the replication cycle. Cis-acting sequences in PNRSV RNA 3 that are recognized by the AMV replicase were studied in in vitro replicase assays and by inoculation of AMV–PNRSV RNA 3 chimeras to tobacco plants and protoplasts transformed with the AMV replicase genes (P12 plants). The results showed that the AMV replicase recognized the promoter for minus-strand RNA synthesis in PNRSV RNA 3 but not the promoter for plus-strand RNA synthesis. A chimeric RNA with PNRSV movement protein and CP genes accumulated in tobacco, which is a non-host for PNRSV.




This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
F. Aparicio, J. A. Sanchez-Navarro, and V. Pallas
In vitro and in vivo mapping of the Prunus necrotic ringspot virus coat protein C-terminal dimerization domain by bimolecular fluorescence complementation
J. Gen. Virol., June 1, 2006; 87(6): 1745 - 1750.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. C. L. Olsthoorn, P. C. J. Haasnoot, and J. F. Bol
Similarities and Differences between the Subgenomic and Minus-Strand Promoters of an RNA Plant Virus
J. Virol., April 15, 2004; 78(8): 4048 - 4053.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. C. L. Olsthoorn and J. F. Bol
Role of an Essential Triloop Hairpin and Flanking Structures in the 3' Untranslated Region of Alfalfa Mosaic Virus RNA in In Vitro Transcription
J. Virol., July 29, 2002; 76(17): 8747 - 8756.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2001 by the Society for General Microbiology.