J Gen Virol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamaguchi, S.
Right arrow Articles by Yuasa, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamaguchi, S.
Right arrow Articles by Yuasa, N.
Agricola
Right arrow Articles by Yamaguchi, S.
Right arrow Articles by Yuasa, N.
Journal of General Virology (2001), 82, 1233-1238.
© 2001 Society for General Microbiology

Animal: DNA Viruses

Identification of a genetic determinant of pathogenicity in chicken anaemia virus

S. Yamaguchi1, T. Imada1, N. Kajib,1, M. Mase1, K. Tsukamoto1, N. Tanimura1 and N. Yuasa1

National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan1

Author for correspondence: Shigeo Yamaguchi. Fax +81 298 38 7760. e-mail syama{at}niah.affrc.go.jp

The molecular basis of pathogenicity of the chicken anaemia virus (CAV) needs to be clarified in order to develop a safe, live virus vaccine. In this study, several high- and low-pathogenic infectious DNA clones were obtained from field virus samples after 12 or 38 passages in MDCC-MSB1 cells. The high-pathogenic clones induced a low haematocrit, low weight gain and high mortality. Nucleotide sequence analyses identified one amino acid, at residue 394 of the VP1 capsid protein, as a major determinant of pathogenicity. To determine the role of this amino acid in pathogenicity, chimeric infectious DNA clones and point-mutated clones were used for chicken pathogenicity tests. These analyses clearly demonstrated that residue 394 of VP1 was crucial for the pathogenicity of CAV; all of the cloned viruses with glutamine at this position were highly pathogenic, whereas those with histidine had low pathogenicity. Low-pathogenic CAV, based on an infectious DNA clone, is a candidate for a genetically homogeneous and stable CAV live vaccine.




This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
T. Opriessnig, N. E. McKeown, E.-M. Zhou, X.-J. Meng, and P. G. Halbur
Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence.
J. Gen. Virol., October 1, 2006; 87(Pt 10): 2923 - 2932.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
K. Kamada, A. Kuroishi, T. Kamahora, P. Kabat, S. Yamaguchi, and S. Hino
Spliced mRNAs detected during the life cycle of Chicken anemia virus.
J. Gen. Virol., August 1, 2006; 87(Pt 8): 2227 - 2233.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Fenaux, T. Opriessnig, P. G. Halbur, F. Elvinger, and X. J. Meng
Two Amino Acid Mutations in the Capsid Protein of Type 2 Porcine Circovirus (PCV2) Enhanced PCV2 Replication In Vitro and Attenuated the Virus In Vivo
J. Virol., December 15, 2004; 78(24): 13440 - 13446.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Todd, A. N. J. Scott, N. W. Ball, B. J. Borghmans, and B. M. Adair
Molecular Basis of the Attenuation Exhibited by Molecularly Cloned Highly Passaged Chicken Anemia Virus Isolates
J. Virol., August 15, 2002; 76(16): 8472 - 8474.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2001 by the Society for General Microbiology.