| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1
Author for correspondence: J. Ignacio Casal. Fax +34 91 4087598. e-mail icasal{at}ingenasa.es
Glycoprotein 5 (GP5) is the major glycoprotein of porcine reproductive and respiratory syndrome virus (PRRSV). Expression of GP5 has been improved by removing the transmembrane regions. Vectors were constructed encoding complete GP5 plus three mutants: GP5 
Ns (residues 28–201), GP5[30–67] (residues 30–67) and GP5[30–201] (residues 30–67/130–201). The three deletion mutants were expressed at levels 20–30 times higher than complete GP5. GP5[30–201] was well recognized in ELISA or immunoblotting by a collection of pig sera. All the fragments were tested for the generation of MAbs, but only the polyhistidine-tagged fragment GP5[30–201]H elicited an antibody response sufficient to produce MAbs. The two MAbs were positive for PRRSV in ELISA and immunoblotting, but negative for virus neutralization. MAb 4BE12 reacted with residues 130–170 and MAb 3AH9 recognized residues 170–201. This region was recognized strongly in immunoblotting by a collection of infected-pig sera. These results indicate diagnostic potential for this epitope.
This article has been cited by other articles:
|
|
 |
|
  S. Costers, P. L. Delputte, and H. J. Nauwynck Porcine reproductive and respiratory syndrome virus-infected alveolar macrophages contain no detectable levels of viral proteins in their plasma membrane and are protected against antibody-dependent, complement-mediated cell lysis. J. Gen. Virol., August 1, 2006; 87(Pt 8): 2341 - 2351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. J. Wissink, M. V. Kroese, H. A. R. van Wijk, F. A. M. Rijsewijk, J. J. M. Meulenberg, and P. J. M. Rottier Envelope Protein Requirements for the Assembly of Infectious Virions of Porcine Reproductive and Respiratory Syndrome Virus J. Virol., October 1, 2005; 79(19): 12495 - 12506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. J. Wissink, H. A. R. van Wijk, M. V. Kroese, E. Weiland, J. J. M. Meulenberg, P. J. M. Rottier, and P. A. van Rijn The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain J. Gen. Virol., June 1, 2003; 84(6): 1535 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Stadejek, A. Stankevicius, T. Storgaard, M. B. Oleksiewicz, S. Belak, T. W. Drew, and Z. Pejsak Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses J. Gen. Virol., August 1, 2002; 83(8): 1861 - 1873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Oleksiewicz, A. Botner, and P. Normann Porcine B-cells recognize epitopes that are conserved between the structural proteins of American- and European-type porcine reproductive and respiratory syndrome virus J. Gen. Virol., June 1, 2002; 83(6): 1407 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ostrowski, J. A. Galeota, A. M. Jar, K. B. Platt, F. A. Osorio, and O. J. Lopez Identification of Neutralizing and Nonneutralizing Epitopes in the Porcine Reproductive and Respiratory Syndrome Virus GP5 Ectodomain J. Virol., April 3, 2002; 76(9): 4241 - 4250. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
