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Journal of General Virology (2001), 82, 1457-1463.
© 2001 Society for General Microbiology

Animal: DNA Viruses

Epstein–Barr virus-encoded protein kinase BGLF4 mediates hyperphosphorylation of cellular elongation factor 1{delta} (EF-1{delta}): EF-1{delta} is universally modified by conserved protein kinases of herpesviruses in mammalian cells

Kentaro Kato1,2, Yasushi Kawaguchi1, Michiko Tanaka1, Mie Igarashi1, Akihiko Yokoyama1, Go Matsuda1, Mikiko Kanamori1, Kaori Nakajima1, Yorihiro Nishimura2, Masayuki Shimojima2, Hang T. T. Phung2, Eiji Takahashi2 and Kanji Hiraib,1

Department of Tumor Virology, Division of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan1
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan2

Author for correspondence: Yasushi Kawaguchi. Fax +81 3 5803 0241. e-mail kawagchi.creg{at}mri.tmd.ac.jp

Translation elongation factor 1{delta} (EF-1{delta}) is hyperphosphorylated in various mammalian cells infected with alpha-, beta- and gammaherpesviruses and EF-1{delta} modification is mediated by viral protein kinases, including UL13 of herpes simplex virus type 1 and UL97 of human cytomegalovirus. In this study, the following is reported. (i) BGLF4 encoded by the prototype gammaherpesvirus Epstein–Barr virus was purified as a fusion protein that was labelled with [{gamma}-32P]ATP and labelling was eliminated by phosphatase. (ii) The ratio of the hyperphosphorylated form of human EF-1{delta} was increased both in Sf9 cells after infection with baculoviruses expressing GST–BGLF4 fusion proteins and in COS-7 cells after transfection with a BGLF4 expression plasmid. These results indicate that purified BGLF4 possesses protein kinase activity and mediates EF-1{delta} hyperphosphorylation. These data also support the hypothesis that the protein kinases that are conserved by herpesviruses universally mediate EF-1{delta} modification in mammalian cells.




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