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Faculty of Physics and Faculty of Chemistry1, Department of Virology and A. N. Belozersky Institute of Physico-Chemical Biology2, Moscow State University, Vorobiovy Gory, Moscow 119899, Russia
Author for correspondence: Josef Atabekov. Fax +7 095 938 06 01. e-mail Atabekov{at}genebee.msu.su
The structure of complexes formed in vitro by tobacco mosaic virus (TMV)-coded movement protein (MP) with TMV RNA and short (890 nt) synthetic RNA transcripts was visualized by atomic force microscopy on a mica surface. MP molecules were found to be distributed along the chain of RNA and the structure of MPRNA complexes depended on the molar MP:RNA ratios at which the complexes were formed. A rise in the molar MP:TMV RNA ratio from 20:1 to 60100:1 resulted in an increase in the density of the MP packaging on TMV RNA and structural conversion of complexes from RNase-sensitive beads-on-a-string into a thick string form that was partly resistant to RNase. The thick string-type RNase-resistant complexes were also produced by short synthetic RNA transcripts at different MP:RNA ratios. The thick string complexes are suggested to represent clusters of MP molecules cooperatively bound to discrete regions of TMV RNA and separated by protein-free RNA segments.
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