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Journal of General Virology (2001), 82, 1529-1532.
© 2001 Society for General Microbiology

Plant

The ORF0 product of Potato leafroll virus is indispensable for virus accumulation

Ewa Sadowy1,2, Anna Maasen1, Marek Juszczuk1, Chantal David2, Wlodzimierz Zagórski-Ostoja1, Bruno Gronenborn2 and M. Danuta Hulanicka1

Institute of Biochemistry and Biophysics PAS, Ul. Pawiskiego 5A, 02-106 Warsaw, Poland1
Institut des Sciences Végétales CNRS, Av. de la Terrasse, 91 198 Gif-sur-Yvette, France2

Author for correspondence: Ewa Sadowy. Present address: Sera & Vaccine Central Research Laboratory, Ul. Chelmska 30/34, 00-725 Warszawa, Poland. Fax +48 22 841 29 49. e-mail sadowy{at}ibbrain.ibb.waw.pl

Using a cDNA expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by ORF0 of Potato leafroll virus (PLRV) and have shown its importance for virus accumulation. Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. They did not, however, affect translation of ORF1 and ORF2. We therefore conclude that ORF0 of PLRV produces a protein essential for virus accumulation, a hitherto undescribed finding.




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