HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cheynier, R. Articles by Wain-Hobson, S. Search for Related Content PubMed PubMed Citation Articles by Cheynier, R. Articles by Wain-Hobson, S. Agricola Articles by Cheynier, R. Articles by Wain-Hobson, S.

Insertion/deletion frequencies match those of point mutations in the hypervariable regions of the simian immunodeficiency virus surface envelope gene

Unité de Rétrovirologie Moléculaire, Institut Pasteur, 28 rue de Dr Roux, F-75724 Paris cedex 15, France¹
Abteilung Virologie, Universität des Saarlandes, Institut für Medizinische Mikrobiologie und Hygiene, D-66421 Homburg, Germany²

Author for correspondence: Simon Wain-Hobson. Fax +33 1 4568 8874. e-mail simon{at}pasteur.fr

A method for encoding insertions and deletions (indels) has been developed and adapted to the SplitsTree program. Following phylogenetic reconstruction, the relative frequencies of indels were estimated for a large number of in vivo sequence sets corresponding to the env V1 hypervariable region of the simian immunodeficiency virus SIVmac251. The method allowed recovery of many point mutations hitherto lost due to gap stripping. Deletions were as frequent as transversions and were 4- to 8-fold more frequent than insertions, invariably duplications. The high proportion of deletions among mutation events suggests that lentivirus vectors may readily delete parts of their cargo.

This article has been cited by other articles:

				G. Bocharov, N. J. Ford, J. Edwards, T. Breinig, S. Wain-Hobson, and A. Meyerhans A genetic-algorithm approach to simulating human immunodeficiency virus evolution reveals the strong impact of multiply infected cells and recombination J. Gen. Virol., November 1, 2005; 86(11): 3109 - 3118. [Abstract] [Full Text] [PDF]

				C. Renoux, S. Wain-Hobson, B. Hurtrel, and R. Cheynier Antigenic Stimulation Specifically Reactivates the Replication of Archived Simian Immunodeficiency Virus Genomes in Chronically Infected Macaques J. Virol., September 1, 2005; 79(17): 11231 - 11238. [Abstract] [Full Text] [PDF]

				K. M. Kitrinos, N. G. Hoffman, J. A. E. Nelson, and R. Swanstrom Turnover of env Variable Region 1 and 2 Genotypes in Subjects with Late-Stage Human Immunodeficiency Virus Type 1 Infection J. Virol., June 15, 2003; 77(12): 6811 - 6822. [Abstract] [Full Text] [PDF]

				S. Wain-Hobson, C. Renoux-Elbe, J.-P. Vartanian, and A. Meyerhans Network analysis of human and simian immunodeficiency virus sequence sets reveals massive recombination resulting in shorter pathways J. Gen. Virol., April 1, 2003; 84(4): 885 - 895. [Abstract] [Full Text] [PDF]

				L. Kils-Hutten, R. Cheynier, S. Wain-Hobson, and A. Meyerhans Phylogenetic reconstruction of intrapatient evolution of human immunodeficiency virus type 1: predominance of drift and purifying selection J. Gen. Virol., July 1, 2001; 82(7): 1621 - 1627. [Abstract] [Full Text] [PDF]