| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
CNRS-FRE2369, Equipe Hépatite C, IBL/Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France1
Author for correspondence: Jean Dubuisson. Fax +33 3 20 87 11 11. e-mail jean.dubuisson{at}ibl.fr
The hepatitis C virus (HCV) genome encodes two envelope glycoproteins, E1 and E2. These proteins contain a large N-terminal ectodomain, and are anchored into membranes by their C-terminal transmembrane domain (TMD). The TMDs of HCV envelope proteins are multifunctional. In addition to their role as membrane anchors, they possess a signal sequence function in their C-terminal half, and play a major role in subcellular localization and assembly of these envelope proteins. In this work, the expression of full-length E2 led to secretion of a proportion of this protein, which is likely to be due to inefficient membrane insertion of a fraction of E2 expressed alone. However, when E1 and E2 were coexpressed from the same polyprotein, E2 was not secreted and remained tightly associated with membranes, suggesting that an early interaction between the TMDs of HCV envelope proteins improves the stability of membrane insertion of E2. These results reinforce the hypothesis that the TMDs of E1 and E2 are major factors in the assembly of the HCV envelope glycoprotein complex.
This article has been cited by other articles:
|
|
 |
|
  G. Sapparapu, S. A. Planque, Y. Nishiyama, S. K. Foung, and S. Paul Antigen-specific Proteolysis by Hybrid Antibodies Containing Promiscuous Proteolytic Light Chains Paired with an Antigen-binding Heavy Chain J. Biol. Chem., September 4, 2009; 284(36): 24622 - 24633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chapel, C. Garcia, B. Bartosch, P. Roingeard, N. Zitzmann, F.-L. Cosset, J. Dubuisson, R. A. Dwek, C. Trepo, F. Zoulim, et al. Reduction of the infectivity of hepatitis C virus pseudoparticles by incorporation of misfolded glycoproteins induced by glucosidase inhibitors J. Gen. Virol., April 1, 2007; 88(4): 1133 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Meertens, C. Bertaux, and T. Dragic Hepatitis C Virus Entry Requires a Critical Postinternalization Step and Delivery to Early Endosomes via Clathrin-Coated Vesicles J. Virol., December 1, 2006; 80(23): 11571 - 11578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Helle, C. Wychowski, N. Vu-Dac, K. R. Gustafson, C. Voisset, and J. Dubuisson Cyanovirin-N Inhibits Hepatitis C Virus Entry by Binding to Envelope Protein Glycans J. Biol. Chem., September 1, 2006; 281(35): 25177 - 25183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chapel, C. Garcia, P. Roingeard, N. Zitzmann, J. Dubuisson, R. A. Dwek, C. Trepo, F. Zoulim, and D. Durantel Antiviral effect of {alpha}-glucosidase inhibitors on viral morphogenesis and binding properties of hepatitis C virus-like particles. J. Gen. Virol., April 1, 2006; 87(Pt 4): 861 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Carrere-Kremer, C. Montpellier, L. Lorenzo, B. Brulin, L. Cocquerel, S. Belouzard, F. Penin, and J. Dubuisson Regulation of Hepatitis C Virus Polyprotein Processing by Signal Peptidase Involves Structural Determinants at the p7 Sequence Junctions J. Biol. Chem., October 1, 2004; 279(40): 41384 - 41392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Flint, C. Logvinoff, C. M. Rice, and J. A. McKeating Characterization of Infectious Retroviral Pseudotype Particles Bearing Hepatitis C Virus Glycoproteins J. Virol., July 1, 2004; 78(13): 6875 - 6882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dumonceaux, E. G. Cormier, F. Kajumo, G. P. Donovan, J. Roy-Chowdhury, I. J. Fox, J. P. Gardner, and T. Dragic Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1 J. Virol., December 15, 2003; 77(24): 13418 - 13424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cocquerel, C.-C. Kuo, J. Dubuisson, and S. Levy CD81-Dependent Binding of Hepatitis C Virus E1E2 Heterodimers J. Virol., October 1, 2003; 77(19): 10677 - 10683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kien, J.-D. Abraham, C. Schuster, and M. P. Kieny Analysis of the subcellular localization of hepatitis C virus E2 glycoprotein in live cells using EGFP fusion proteins J. Gen. Virol., March 1, 2003; 84(3): 561 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cocquerel, E. R. Quinn, M. Flint, K. G. Hadlock, S. K. H. Foung, and S. Levy Recognition of Native Hepatitis C Virus E1E2 Heterodimers by a Human Monoclonal Antibody J. Virol., January 15, 2003; 77(2): 1604 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Op De Beeck, L. Cocquerel, and J. Dubuisson Biogenesis of hepatitis C virus envelope glycoproteins J. Gen. Virol., November 1, 2001; 82(11): 2589 - 2595. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
