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Animal: RNA Viruses |
Department of Microbiology, University of Alabama at Birmingham, BBRB 373/17, 845 19th Street South, Birmingham, AL 35294-2170, USA1
Department of Animal Health and Biomedical Science, University of Wisconsin-Madison, Madison, WI 53706, USA2
Author for correspondence: Andrew Ball. Fax +1 205 934 1636. e-mail andyb{at}uab.edu
The Nodaviridae are a family of isometric RNA viruses that infect insects and fish. Their genomes, which are among the smallest known for animal viruses, consist of two co-encapsidated positive-sense RNA segments: RNA1 encodes the viral contribution to the RNA-dependent RNA polymerase (RdRp) which replicates the viral genome, whereas RNA2 encodes the capsid protein precursor. In this study, the RNA1 sequences of two insect nodaviruses – Nodamura virus (the prototype of the genus) and Boolarra virus – are reported as well as detailed comparisons of their encoded RdRps with those of three other nodaviruses of insects and one of fish. Although the 5' and 3' untranslated regions did not reveal common features of RNA sequence or secondary structure, these divergent viruses showed similar genome organizations and encoded RdRps that had from 26 to 99% amino acid sequence identity. All six RdRp amino acid sequences contained canonical RNA polymerase motifs in their C-terminal halves and conserved elements of predicted secondary structure throughout. A search for structural homologues in the protein structure database identified the poliovirus RdRp, 3Dpol, as the best template for homology modelling of the RNA polymerase domain of Pariacoto virus and allowed the construction of a congruent three-dimensional model. These results extend our understanding of the relationships among the RNA1 segments of nodaviruses and the predicted structures of their encoded RdRps.
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B. D. Price, L. D. Eckerle, L. A. Ball, and K. L. Johnson Nodamura Virus RNA Replication in Saccharomyces cerevisiae: Heterologous Gene Expression Allows Replication-Dependent Colony Formation J. Virol., January 1, 2005; 79(1): 495 - 502. [Abstract] [Full Text] [PDF]
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K. N. Johnson, L. Tang, J. E. Johnson, and L. A. Ball Heterologous RNA Encapsidated in Pariacoto Virus-Like Particles Forms a Dodecahedral Cage Similar to Genomic RNA in Wild-Type Virions J. Virol., October 15, 2004; 78(20): 11371 - 11378. [Abstract] [Full Text] [PDF]
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 W.-X. Li, H. Li, R. Lu, F. Li, M. Dus, P. Atkinson, E. W. A. Brydon, K. L. Johnson, A. Garcia-Sastre, L. A. Ball, et al. Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing PNAS, February 3, 2004; 101(5): 1350 - 1355. [Abstract] [Full Text] [PDF]
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T. Iwamoto, Y. Okinaka, K. Mise, K.-I. Mori, M. Arimoto, T. Okuno, and T. Nakai Identification of Host-Specificity Determinants in Betanodaviruses by Using Reassortants between Striped Jack Nervous Necrosis Virus and Sevenband Grouper Nervous Necrosis Virus J. Virol., February 1, 2004; 78(3): 1256 - 1262. [Abstract] [Full Text] [PDF]
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K. N. Johnson and L. A. Ball Virions of Pariacoto virus contain a minor protein translated from the second AUG codon of the capsid protein open reading frame J. Gen. Virol., October 1, 2003; 84(10): 2847 - 2852. [Abstract] [Full Text] [PDF]
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B. D. Price, P. Ahlquist, and L. A. Ball DNA-Directed Expression of an Animal Virus RNA for Replication-Dependent Colony Formation in Saccharomyces cerevisiae J. Virol., February 15, 2002; 76(4): 1610 - 1616. [Abstract] [Full Text] [PDF]
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K. N. Johnson and L. A. Ball Recovery of Infectious Pariacoto Virus from cDNA Clones and Identification of Susceptible Cell Lines J. Virol., December 15, 2001; 75(24): 12220 - 12227. [Abstract] [Full Text] [PDF]
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