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Animal: RNA Viruses |
by human peripheral blood mononuclear cells in vitro
Laboratoire de Virologie, Centre Hospitalier Régional et Universitaire, Institut Gernez-Rieux, 59037 Lille Cedex, France1
SEDAC Therapeutics, IBL–CNRS, 59021 Lille, France2
Department of Veterinary Immunology, Biomedical Center, Uppsala, Sweden3
Author for correspondence: Didier Hober. Fax +33 3 20 44 52 81. e-mail dhober{at}chru-lille.fr
Coxsackievirus B4 (CVB4) can be found in circulating blood of patients; however, the interaction of CVB4 with peripheral blood mononuclear cells (PBMCs) is poorly understood. CVB4 induced low levels of IFN-
synthesis in PBMCs from healthy donors. In contrast, preincubation of infectious CVB4 with plasma from these donors containing anti-CVB4 antibodies strongly enhanced the synthesis of IFN-. IgG obtained from plasma by chromatography formed immune complexes with CVB4 and increased significantly the CVB4-induced production of IFN- by PBMCs. These antibodies did not have a neutralizing effect on CVB4 infection of Hep-2 cells. The role of CVB and adenovirus receptor (CAR), Fc
RII and FcRIII in the increased synthesis of IFN- induced by CVB4 preincubated with IgG was shown by inhibition with specific antibodies. The major interferon--producing cells in response to CVB4–IgG complexes were CD14+ cells and monocyte-enriched PBMCs. With the latter, detection of IFN- by immunostaining was positive whereas in monocyte-depleted PBMCs it was not. This study shows that CVB4-induced synthesis of IFN- by PBMCs can be enhanced by an antibody-dependent mechanism through interactions between the virus, non-neutralizing antivirus antibodies, FcRII and III and CAR.
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