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Cys⁹, Cys¹⁰⁴ and Cys²⁰⁷ of simian virus 40 Vp1 are essential for infectious virion formation in CV-1 cells

Department of Biological Sciences, California State University at Long Beach, 1250 Bellflower Blvd, Long Beach, CA 90840-3702, USA¹

Author for correspondence: Editte Gharakhanian. Fax +1 562 985 8878. e-mail eghara{at}csulb.edu

Structural studies have implicated Cys⁹, Cys¹⁰⁴ and Cys²⁰⁷ of simian virus 40 (SV40) Vp1 in disulfide bond formation. Recently, we have shown the three cysteines to be essential for disulfide linkage of Vp1 complexes in vitro. Here, the role of the three cysteines was explored during the course of SV40 infection. Single-, double- and triple-mutant Vp1 at Cys⁹, Cys¹⁰⁴ and Cys²⁰⁷ continued to localize to the nuclei of transfected CV-1 cells and to bind DNA, but showed a range of abilities to form plaques. Only mutants containing the Cys⁹Ser change showed defects in plaque formation. Single mutants at Cys⁹ formed small plaques; mutants at Cys⁹ . Cys¹⁰⁴, Cys⁹ . Cys²⁰⁷ and Cys⁹ . Cys¹⁰⁴ . Cys²⁰⁷ formed no plaques. All three isolated revertants contained back-mutations at the Vp1 Cys⁹ codon. These results further confirm the involvement of the three Vp1 cysteines in protein–protein interactions during virus assembly. Cys⁹ is critical for production of wild-type infectious virions, whereas Cys¹⁰⁴ and Cys²⁰⁷ play secondary roles.

This article has been cited by other articles:

				P. P. Li, A. Nakanishi, V. Fontanes, and H. Kasamatsu Pairs of Vp1 Cysteine Residues Essential for Simian Virus 40 Infection J. Virol., March 15, 2005; 79(6): 3859 - 3864. [Abstract] [Full Text] [PDF]