J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Topham, D. J.
Right arrow Articles by Doherty, P. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Topham, D. J.
Right arrow Articles by Doherty, P. C.
Agricola
Right arrow Articles by Topham, D. J.
Right arrow Articles by Doherty, P. C.
Journal of General Virology (2001), 82, 1971-1981.
© 2001 Society for General Microbiology

Animal: DNA Viruses

Perforin and Fas in murine gammaherpesvirus-specific CD8+ T cell control and morbidity

David J. Topham1, Rhonda C. Cardinb,1, Jan P. Christensenc,1, James W. Brooksd,1, Gabrielle T. Belze,1 and Peter C. Doherty1

Department of Immunology, St Jude Children’s Research Hospital, 332 North Lauderdale, Memphis, TN 38101, USA1

Author for correspondence: David Topham. Present address: David H. Smith Center for Vaccine Biology & Immunology, Aab Institute for Biomedical Sciences, University of Rochester Medical Center, 601 Elmwood Avenue, Box 609, Rochester, NY 14642-8609, USA. Fax +1 716 273 2452. e-mail david_topham{at}urmc.rochester.edu

The immune system uses both virus-specific T cells and B cells to control the acute and latent phases of respiratory infection with the murine gammaherpesvirus 68 ({gamma}HV-68). We sought to further define the important effector mechanisms for CD8+ T cells. First, depletion of the CD4+ T cells resulted in a failure of most animals to drive the virus into latency, although lytic virus in the lung was reduced by approximately 1000-fold from its peak. Second, the absence of either perforin or Fas alone had no impact on the ability to reduce titres of lytic virus in the lung. Further neutralization of IFN-{gamma} in CD4-depleted P+/+, P-/- or Fas-/- mice had no effect. To define the requirements for Fas or perforin more clearly, two sets of chimeric mice were constructed differing in perforin expression by the T cells, and Fas on infected epithelial cells or lymphocytes. Animals with P-/- T cells and a Fas-/- lung failed to limit the shedding of infectious virus, regardless of whether CD4 T cells were present. In addition, we noted that having P-/- T cells in irradiated Fas+/+ hosts caused a lethal disease that was not apparent in the non-chimeric (unirradiated) P-/- (Fas+/+) mice. In another set of chimeric mice, P-/- T cells were able to limit persistent infection of B cells that expressed Fas, but not B cells that were Fas-deficient. These studies demonstrate that some degree of cytotoxicity via either perforin or Fas is essential for CD8+ T cells to control this DNA virus.




This article has been cited by other articles:


Home page
 J. Virol.Home page
E. S. Barton, M. L. Lutzke, R. Rochford, and H. W. Virgin IV
Alpha/Beta Interferons Regulate Murine Gammaherpesvirus Latent Gene Expression and Reactivation from Latency
J. Virol., November 15, 2005; 79(22): 14149 - 14160.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. J. Lee, F. Giannoni, A. Lyon, S. Yada, B. Lu, C. Gerard, and S. R. Sarawar
Role of CXCR3 in the Immune Response to Murine Gammaherpesvirus 68
J. Virol., July 15, 2005; 79(14): 9351 - 9355.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Giannoni, A. B. Lyon, M. D. Wareing, P. B. Dias, and S. R. Sarawar
Protein Kinase C {theta} Is Not Essential for T-Cell-Mediated Clearance of Murine Gammaherpesvirus 68
J. Virol., June 1, 2005; 79(11): 6808 - 6813.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
A. B. Kamath, J. Woodworth, X. Xiong, C. Taylor, Y. Weng, and S. M. Behar
Cytolytic CD8+ T Cells Recognizing CFP10 Are Recruited to the Lung after Mycobacterium tuberculosis Infection
J. Exp. Med., December 6, 2004; 200(11): 1479 - 1489.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. B. Jordan, D. Hildeman, J. Kappler, and P. Marrack
An animal model of hemophagocytic lymphohistiocytosis (HLH): CD8+ T cells and interferon gamma are essential for the disorder
Blood, August 1, 2004; 104(3): 735 - 743.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Andreansky, H. Liu, H. Adler, U. H. Koszinowski, S. Efstathiou, and P. C. Doherty
The limits of protection by "memory" T cells in Ig-/- mice persistently infected with a {gamma}-herpesvirus
PNAS, February 17, 2004; 101(7): 2017 - 2022.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Liu, S. Andreansky, G. Diaz, T. Hogg, and P. C. Doherty
Reduced Functional Capacity of CD8+ T Cells Expanded by Post-Exposure Vaccination of {gamma}-Herpesvirus-Infected CD4-Deficient Mice
J. Immunol., April 1, 2002; 168(7): 3477 - 3483.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. M. Licon Luna, E. Lee, A. Mullbacher, R. V. Blanden, R. Langman, and M. Lobigs
Lack of both Fas Ligand and Perforin Protects from Flavivirus-Mediated Encephalitis in Mice
J. Virol., March 7, 2002; 76(7): 3202 - 3211.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2001 by the Society for General Microbiology.