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Distribution of rotavirus-specific memory B cells in gut-associated lymphoid tissue after primary immunization

Section of Infectious Diseases, The Children’s Hospital of Philadelphia, Abramson Research Building, Room 1205A, 3516 Civic Center Blvd, Philadelphia, PA 19104, USA¹
The University of Pennsylvania School of Medicine² and The Wistar Institute of Anatomy and Biology³, Philadelphia, PA 19104, USA

Author for correspondence: Charlotte Moser. Fax +1 215 590 2025. e-mail moser{at}email.chop.edu

We found previously that mice inoculated orally with simian rotavirus strain RRV developed virus-specific memory B cell responses 16 weeks after immunization that were greater than those found 6 weeks after immunization. Memory B cell responses were defined as the quantity of virus-specific IgA detected in small intestinal lamina propria (LP) fragment cultures of immunized mice at various intervals after challenge. Enhanced memory B cell responses correlated with enhanced protection against shedding. In order to understand better the delayed onset of rotavirus-specific memory B cell responses, a method was developed to determine the frequencies of rotavirus-specific memory B cells in gut-associated lymphoid tissues (GALT). We found that protection against rotavirus challenge was determined by the frequency of rotavirus-specific memory B cells in GALT LP.