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Animal: DNA Viruses |
ke Liljeqvist1
Department of Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden1
UPR9021 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France2
Author for correspondence: Jan-ke Liljeqvist. Fax +46 31 3424960. e-mail jan-ake.liljeqvist{at}microbio.gu.se
Glycoprotein G-2 (gG-2) of herpes simplex virus type 2 (HSV-2) is cleaved to a secreted amino-terminal portion (sgG-2) and to a cell-associated carboxy-terminal portion which is further O-glycosylated to constitute the mature gG-2 (mgG-2). In contrast to mgG-2, which is known to elicit a type-specific antibody response in the human host, information on the immunogenic properties of sgG-2 is lacking. Here the sgG-2 protein was purified on a heparin column and used for production of monoclonal antibodies (mAbs). Four anti-sgG-2 mAbs were mapped using a Pepscan technique and identified linear epitopes which localized to the carboxy-terminal part of the protein. One additional anti-sgG-2 mAb, recognizing a non-linear epitope, was reactive to three discrete peptide stretches where the most carboxy-terminally located stretch was constituted by the amino acids 320RRAL323. Although sgG-2 is rapidly secreted into the cell-culture medium after infection, the anti-sgG-2 mAbs identified substantial amounts of sgG-2 in the cytoplasm of HSV-2-infected cells. All of the anti-sgG-2 mAbs were HSV-2 specific showing no cross-reactivity to HSV-1 antigen or to HSV-1-infected cells. Similarly, sera from 50 HSV-2 isolation positive patients were all reactive to sgG-2 in an enzyme immunoassay whilst no reactivity was seen in 25 sera from HSV-1 isolation positive patients or in 25 serum samples from HSV-negative patients suggesting that sgG-2 is a novel antigen potentially suitable for type-discriminating serodiagnosis.
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 P. Tunback, T. Bergstrom, G.-B. Lowhagen, J. Hoebeke, and J.-A. Liljeqvist Type-specific reactivity of anti-glycoprotein G antibodies from herpes simplex virus-infected individuals is maintained by single or dual type-specific residues J. Gen. Virol., February 1, 2005; 86(2): 247 - 251. [Abstract] [Full Text] [PDF]
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K. Eriksson, L. Bellner, S. Gorander, G.-B. Lowhagen, P. Tunback, K. Rydberg, and J.-A. Liljeqvist CD4+ T-cell responses to herpes simplex virus type 2 (HSV-2) glycoprotein G are type specific and differ in symptomatic and asymptomatic HSV-2-infected individuals J. Gen. Virol., August 1, 2004; 85(8): 2139 - 2147. [Abstract] [Full Text] [PDF]
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S. Gorander, B. Svennerholm, and J.-A. Liljeqvist Secreted Portion of Glycoprotein G of Herpes Simplex Virus Type 2 Is a Novel Antigen for Type-Discriminating Serology J. Clin. Microbiol., August 1, 2003; 41(8): 3681 - 3686. [Abstract] [Full Text] [PDF]
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