J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow Corrigendum (v83,p1251)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Law, M.
Right arrow Articles by Smith, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Law, M.
Right arrow Articles by Smith, G. L.
Agricola
Right arrow Articles by Law, M.
Right arrow Articles by Smith, G. L.
Journal of General Virology (2002), 83, 209-222.
© 2002 Society for General Microbiology

Animal: DNA Viruses

Antibody-sensitive and antibody-resistant cell-to-cell spread by vaccinia virus: role of the A33R protein in antibody-resistant spread

Mansun Law1, Ruth Hollinshead1 and Geoffrey L. Smith1

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK1

Author for correspondence: Geoffrey Smith. Present address: Wright–Fleming Institute, Imperial College School of Medicine, St Mary’s Campus, Norfolk Place, London W2 1PG, UK. Fax +44 20 7594 3973. e-mail glsmith{at}ic.ac.uk

The roles of vaccinia virus (VV) intracellular mature virus (IMV), intracellular enveloped virus (IEV), cell-associated enveloped virus (CEV) and extracellular enveloped virus (EEV) and their associated proteins in virus spread were investigated. The plaques made by VV mutants lacking individual IEV- or EEV-specific proteins (v{Delta}A33R, v{Delta}A34R, v{Delta}A36R, v{Delta}A56R, v{Delta}B5R, v{Delta}F12L and v{Delta}F13L) were compared in the presence of IMV- or EEV-neutralizing antibodies (Ab). Data presented show that for long-range spread, the comet-shaped plaques of VV were caused by the unidirectional spread of EEV probably by convection currents, and for cell-to-cell spread, VV uses a combination of Ab-resistant and Ab-sensitive pathways. Actin tails play a major role in the Ab-resistant pathway, but mutants such as v{Delta}A34R and v{Delta}A36R that do not make actin tails still spread from cell to cell in the presence of Ab. Most strikingly, the Ab-resistant pathway was abolished when the A33R gene was deleted. This effect was not due to alterations in the efficiency of neutralization of EEV made by this mutant, nor due to a deficiency in IMV wrapping to form IEV, which was indispensable for EEV formation by v{Delta}A33R and v{Delta}A34R. We suggest a role for A33R in promoting Ab-resistant cell-to-cell spread of virus. The roles of the different virus forms in the VV life-cycle are discussed.




This article has been cited by other articles:


Home page
 J. Virol.Home page
C. N. Fogg, J. L. Americo, P. L. Earl, W. Resch, L. Aldaz-Carroll, R. J. Eisenberg, G. H. Cohen, and B. Moss
Disparity between Levels of In Vitro Neutralization of Vaccinia Virus by Antibody to the A27 Protein and Protection of Mice against Intranasal Challenge
J. Virol., August 15, 2008; 82(16): 8022 - 8029.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. R. Kaufman, J. Goudsmit, L. Holterman, B. A. Ewald, M. Denholtz, C. Devoy, A. Giri, L. E. Grandpre, J.-M. Heraud, G. Franchini, et al.
Differential Antigen Requirements for Protection against Systemic and Intranasal Vaccinia Virus Challenges in Mice
J. Virol., July 15, 2008; 82(14): 6829 - 6837.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. H. Kirn, Y. Wang, W. Liang, C. H. Contag, and S. H. Thorne
Enhancing Poxvirus Oncolytic Effects through Increased Spread and Immune Evasion
Cancer Res., April 1, 2008; 68(7): 2071 - 2075.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Z. Chen, P. Earl, J. Americo, I. Damon, S. K. Smith, F. Yu, A. Sebrell, S. Emerson, G. Cohen, R. J. Eisenberg, et al.
Characterization of Chimpanzee/Human Monoclonal Antibodies to Vaccinia Virus A33 Glycoprotein and Its Variola Virus Homolog In Vitro and in a Vaccinia Virus Mouse Protection Model
J. Virol., September 1, 2007; 81(17): 8989 - 8995.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
A. D. Garcia, C. A. Meseda, A. E. Mayer, A. Kumar, M. Merchlinsky, and J. P. Weir
Characterization and Use of Mammalian-Expressed Vaccinia Virus Extracellular Membrane Proteins for Quantification of the Humoral Immune Response to Smallpox Vaccines
Clin. Vaccine Immunol., August 1, 2007; 14(8): 1032 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Chen, P. Earl, J. Americo, I. Damon, S. K. Smith, Y.-H. Zhou, F. Yu, A. Sebrell, S. Emerson, G. Cohen, et al.
Chimpanzee/human mAbs to vaccinia virus B5 protein neutralize vaccinia and smallpox viruses and protect mice against vaccinia virus
PNAS, February 7, 2006; 103(6): 1882 - 1887.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Lustig, C. Fogg, J. C. Whitbeck, R. J. Eisenberg, G. H. Cohen, and B. Moss
Combinations of Polyclonal or Monoclonal Antibodies to Proteins of the Outer Membranes of the Two Infectious Forms of Vaccinia Virus Protect Mice against a Lethal Respiratory Challenge
J. Virol., November 1, 2005; 79(21): 13454 - 13462.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M. Law, M. Hollinshead, H.-J. Lee, and G. L. Smith
Yaba-like disease virus protein Y144R, a member of the complement control protein family, is present on enveloped virions that are associated with virus-induced actin tails
J. Gen. Virol., May 1, 2004; 85(5): 1279 - 1290.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Funk, H. Hohenberg, M. Mhamdi, H. Will, and H. Sirma
Spread of Hepatitis B Viruses In Vitro Requires Extracellular Progeny and May Be Codetermined by Polarized Egress
J. Virol., April 15, 2004; 78(8): 3977 - 3983.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. S. Wyatt, P. L. Earl, L. A. Eller, and B. Moss
Highly attenuated smallpox vaccine protects mice with and without immune deficiencies against pathogenic vaccinia virus challenge
PNAS, March 30, 2004; 101(13): 4590 - 4595.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
G. C. Carter, G. Rodger, B. J. Murphy, M. Law, O. Krauss, M. Hollinshead, and G. L. Smith
Vaccinia virus cores are transported on microtubules
J. Gen. Virol., September 1, 2003; 84(9): 2443 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M. Pires de Miranda, P. C. Reading, D. C. Tscharke, B. J. Murphy, and G. L. Smith
The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model
J. Gen. Virol., September 1, 2003; 84(9): 2459 - 2471.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
G. L. Smith, A. Vanderplasschen, and M. Law
The formation and function of extracellular enveloped vaccinia virus
J. Gen. Virol., December 1, 2002; 83(12): 2915 - 2931.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
O. Krauss, R. Hollinshead, M. Hollinshead, and G. L. Smith
An investigation of incorporation of cellular antigens into vaccinia virus particles
J. Gen. Virol., October 1, 2002; 83(10): 2347 - 2359.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2002 by the Society for General Microbiology.