HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nishihara, T. Search for Related Content PubMed PubMed Citation Articles by Nishihara, T. Agricola Articles by Nishihara, T.

Various morphological aspects of Escherichia coli lysis by two distinct RNA bacteriophages

Department of Biochemistry, Kawasaki Medical School, Kurashiki, Japan¹

Author for correspondence: Tohru Nishihara. Present address: The West Field Institute, Showa 2-chome, 4-23-1101, Kurashiki City, Okayama 710-0057, Japan. Fax +81 86 421 3409. e-mail tnishihara{at}mf.0038.net

Transmission electron micrographs of Escherichia coli cells induced by cloned lysis genes from RNA bacteriophages GA (group A-II) and SP (group B-IV) revealed various morphological aspects of intermediates of lysing cells. Cells induced by the SP lysis gene became stretched and also tapered in shape and fragmentation of parts of the cells had also occurred. Cells induced by the GA lysis gene showed many ballooning structures on the cell surfaces and others leaked material through the cell wall. Some balloon-like structures also appeared on the surfaces of cells induced by the cloned lysis gene of RNA phage SP and material also appeared to be leaking through the cell wall in the photographs. The lysing cells observed by transmission electron microscopy showed various morphological aspects of intermediates of the lysing process.

This article has been cited by other articles:

				T. Nishihara, H. Morisawa, N. Ohta, J. F Atkins, and Y. Nishimura A cryptic lysis gene near the start of the Q{beta} replicase gene in the +1 frame Genes Cells, October 1, 2004; 9(10): 877 - 889. [Abstract] [Full Text] [PDF]