J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jeffs, S. A.
Right arrow Articles by McKeating, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jeffs, S. A.
Right arrow Articles by McKeating, J. A.
Agricola
Right arrow Articles by Jeffs, S. A.
Right arrow Articles by McKeating, J. A.
Journal of General Virology (2002), 83, 2723-2732.
© 2002 Society for General Microbiology

Animal: RNA Viruses

Truncated gp120 envelope glycoprotein of human immunodeficiency virus 1 elicits a broadly reactive neutralizing immune response

S. A. Jeffs1, C. Shotton2, P. Balfe3 and J. A. McKeating4

Division of Retrovirology, NIBSC, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK1
School of Biological Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK2
Division of Virology, University College of London Medical School, Windeyer Building, 46 Cleveland Street, London W1P 6DB, UK3
School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading, Berks RG6 2AJ, UK4

Author for correspondence: Simon Jeffs. Fax +44 1707 649865. e-mail sjeffs{at}nibsc.ac.uk

Removal of the V1–V3 loops from IIIB gp120 results in a protein, PR12, with altered immunogenicity compared to the full-length protein. Polyclonal immune sera raised in rats using PR12 as immunogen recognizes envelope glycoproteins of clades A, B, C, E, F and G and can neutralize chimeric human immunodeficiency virus type 1 (HIV-1) HXB2 viruses expressing envelopes from primary HIV-1 clades B, C, E and F. These data suggest that the immune response to PR12 is directed toward conserved epitopes expressed by viral glycoproteins of diverse genotypes. Five monoclonal antibodies (mAb) derived from PR12-immunized rats were unable to neutralize virus infectivity; hence the epitopes responsible for the induction of this cross-clade neutralizing activity remain to be elucidated. However, PR12 immune sera were able to compete with the human neutralizing mAb 2G12 for gp120 binding, implying that this epitope may be immunogenic when expressed in the context of this truncated protein.




This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
M. Montero, N. E. van Houten, X. Wang, and J. K. Scott
The Membrane-Proximal External Region of the Human Immunodeficiency Virus Type 1 Envelope: Dominant Site of Antibody Neutralization and Target for Vaccine Design
Microbiol. Mol. Biol. Rev., March 1, 2008; 72(1): 54 - 84.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. K. Gorny, C. Williams, B. Volsky, K. Revesz, X.-H. Wang, S. Burda, T. Kimura, F. A. J. Konings, A. Nadas, C. A. Anyangwe, et al.
Cross-Clade Neutralizing Activity of Human Anti-V3 Monoclonal Antibodies Derived from the Cells of Individuals Infected with Non-B Clades of Human Immunodeficiency Virus Type 1
J. Virol., July 15, 2006; 80(14): 6865 - 6872.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. Poon, J. F. Hsu, V. Gudeman, I. S. Y. Chen, and K. Grovit-Ferbas
Formaldehyde-Treated, Heat-Inactivated Virions with Increased Human Immunodeficiency Virus Type 1 Env Can Be Used To Induce High-Titer Neutralizing Antibody Responses
J. Virol., August 15, 2005; 79(16): 10210 - 10217.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. Poon, J. T. Safrit, H. McClure, C. Kitchen, J. F. Hsu, V. Gudeman, C. Petropoulos, T. Wrin, I. S. Y. Chen, and K. Grovit-Ferbas
Induction of Humoral Immune Responses following Vaccination with Envelope-Containing, Formaldehyde-Treated, Thermally Inactivated Human Immunodeficiency Virus Type 1
J. Virol., April 15, 2005; 79(8): 4927 - 4935.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. D. Trujillo, N. M. Kumpula-McWhirter, K. J. Hotzel, M. Gonzalez, and W. P. Cheevers
Glycosylation of Immunodominant Linear Epitopes in the Carboxy-Terminal Region of the Caprine Arthritis-Encephalitis Virus Surface Envelope Enhances Vaccine-Induced Type-Specific and Cross-Reactive Neutralizing Antibody Responses
J. Virol., September 1, 2004; 78(17): 9190 - 9202.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M.-Y. Zhang, X. Xiao, I. A. Sidorov, V. Choudhry, F. Cham, P. F. Zhang, P. Bouma, M. Zwick, A. Choudhary, D. C. Montefiori, et al.
Identification and Characterization of a New Cross-Reactive Human Immunodeficiency Virus Type 1-Neutralizing Human Monoclonal Antibody
J. Virol., September 1, 2004; 78(17): 9233 - 9242.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Lorin, L. Mollet, F. Delebecque, C. Combredet, B. Hurtrel, P. Charneau, M. Brahic, and F. Tangy
A Single Injection of Recombinant Measles Virus Vaccines Expressing Human Immunodeficiency Virus (HIV) Type 1 Clade B Envelope Glycoproteins Induces Neutralizing Antibodies and Cellular Immune Responses to HIV
J. Virol., January 1, 2004; 78(1): 146 - 157.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2002 by the Society for General Microbiology.