HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mankertz, A. Articles by Hillenbrand, B. Search for Related Content PubMed PubMed Citation Articles by Mankertz, A. Articles by Hillenbrand, B. Agricola Articles by Mankertz, A. Articles by Hillenbrand, B.

Analysis of transcription of Porcine circovirus type 1

P24 (Xenotransplantation), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany¹

Author for correspondence: Annette Mankertz. Fax +49 1888 754 2598. e-mail mankertza{at}rki.de

Porcine circovirus type 1 (PCV1) contains two major open reading frames encoding the replication initiator proteins, Rep and Rep', and the structural protein, Cap. The promoters of these two genes (P_cap and P_rep) have been mapped. P_cap is located within the rep open reading frame (nt 1328–1252). P_rep has been mapped to the intergenic region immediately upstream of the rep gene (nt 640–796) and overlaps the origin of replication of PCV1. Although binding of both rep gene products to a fragment containing P_rep and the overlapping origin of replication has been reported, only the full-length Rep protein repressed P_rep, while the spliced isoform Rep' did not. P_rep repression is mediated by binding of the Rep protein to the two inner hexamers, H1 and H2, located in the origin of PCV1, whereas binding of Rep to hexamers H3 and H4 was not necessary. Use of Rep mutants indicated that the conserved rolling-circle replication domain II as well as the P loop are essential for repression of P_rep. In contrast to P_rep, transcription of P_cap was not influenced by viral proteins. Additionally, the ratio of the rep and rep' transcripts was analysed. Twelve hours after transfection of PK15 cells with an infectious clone of PCV1, similar amounts of both transcripts were detected, but later the amount of the two transcripts varied, indicating a balanced expression of the two rep transcripts.

This article has been cited by other articles:

				T. Steinfeldt, T. Finsterbusch, and A. Mankertz Functional Analysis of cis- and trans-Acting Replication Factors of Porcine Circovirus Type 1 J. Virol., June 1, 2007; 81(11): 5696 - 5704. [Abstract] [Full Text] [PDF]

				T. Steinfeldt, T. Finsterbusch, and A. Mankertz Demonstration of nicking/joining activity at the origin of DNA replication associated with the rep and rep' proteins of porcine circovirus type 1. J. Virol., July 1, 2006; 80(13): 6225 - 6234. [Abstract] [Full Text] [PDF]

				A. Mankertz, B. Mueller, T. Steinfeldt, C. Schmitt, and T. Finsterbusch New Reporter Gene-Based Replication Assay Reveals Exchangeability of Replication Factors of Porcine Circovirus Types 1 and 2 J. Virol., September 15, 2003; 77(18): 9885 - 9893. [Abstract] [Full Text] [PDF]