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Animal: DNA Viruses |
School of Biological Sciences, University of Nebraska, Lincoln NE 68588, USA1
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, USA2
Author for correspondence: Clinton Jones. Fax +1 402 472 9690. e-mail cjones{at}unlnotes.unl.edu
Transfection of bovine cells with bovine herpesvirus-1 genomic DNA yields low levels of infectious virus. Cotransfection with the bICP0 gene enhances productive infection and virus yield because bICP0 can activate viral gene expression. Since the latency-related (LR) gene overlaps and is antisense to bICP0, the effects of LR gene products on productive infection were tested. The intact LR gene inhibited productive infection in a dose-dependent fashion but LR protein expression was not required. Further studies indicated that LR gene sequences near the 3' terminus of the LR RNA are necessary for inhibiting productive infection. When cotransfected with the bICP0 gene, the LR gene inhibited bICP0 RNA and protein expression in transiently transfected cells. Taken together, these results suggest that abundant LR RNA expression in sensory neurons is one factor that has the potential to inhibit productive infection and consequently promote the establishment and maintenance of latency.
This article has been cited by other articles:
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  A. Workman, S. Perez, A. Doster, and C. Jones Dexamethasone Treatment of Calves Latently Infected with Bovine Herpesvirus 1 Leads to Activation of the bICP0 Early Promoter, in Part by the Cellular Transcription Factor C/EBP-Alpha J. Virol., September 1, 2009; 83(17): 8800 - 8809. [Abstract] [Full Text] [PDF]
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W. Shen and C. Jones Open Reading Frame 2, Encoded by the Latency-Related Gene of Bovine Herpesvirus 1, Has Antiapoptotic Activity in Transiently Transfected Neuroblastoma Cells J. Virol., November 1, 2008; 82(21): 10940 - 10945. [Abstract] [Full Text] [PDF]
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 S. Perez, F. Meyer, K. Saira, A. Doster, and C. Jones Premature expression of the latency-related RNA encoded by bovine herpesvirus type 1 correlates with higher levels of beta interferon RNA expression in productively infected cells J. Gen. Virol., June 1, 2008; 89(6): 1338 - 1345. [Abstract] [Full Text] [PDF]
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F. Meyer, S. Perez, V. Geiser, M. Sintek, M. Inman, and C. Jones A Protein Encoded by the Bovine Herpesvirus 1 Latency-Related Gene Interacts with Specific Cellular Regulatory Proteins, Including CCAAT Enhancer Binding Protein Alpha J. Virol., January 1, 2007; 81(1): 59 - 67. [Abstract] [Full Text] [PDF]
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Y. Zhang, Y. Jiang, V. Geiser, J. Zhou, and C. Jones Bovine herpesvirus 1 immediate-early protein (bICP0) interacts with the histone acetyltransferase p300, which stimulates productive infection and gC promoter activity J. Gen. Virol., July 1, 2006; 87(7): 1843 - 1851. [Abstract] [Full Text] [PDF]
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L. Jin, G.-C. Perng, K. R. Mott, N. Osorio, J. Naito, D. J. Brick, D. Carpenter, C. Jones, and S. L. Wechsler A Herpes Simplex Virus Type 1 Mutant Expressing a Baculovirus Inhibitor of Apoptosis Gene in Place of Latency-Associated Transcript Has a Wild-Type Reactivation Phenotype in the Mouse J. Virol., October 1, 2005; 79(19): 12286 - 12295. [Abstract] [Full Text] [PDF]
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G. Delhon, M. P. Moraes, Z. Lu, C. L. Afonso, E. F. Flores, R. Weiblen, G. F. Kutish, and D. L. Rock Genome of Bovine Herpesvirus 5 J. Virol., October 1, 2003; 77(19): 10339 - 10347. [Abstract] [Full Text] [PDF]
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V. Geiser and C. Jones Stimulation of bovine herpesvirus-1 productive infection by the adenovirus E1A gene and a cell cycle regulatory gene, E2F-4 J. Gen. Virol., April 1, 2003; 84(4): 929 - 938. [Abstract] [Full Text] [PDF]
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