| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Department of Microbiology and Institute of Microbiology and Immunology, National Yang-Ming University, 155 Li-Nong Street Sec. 2, Shih-Pai, Taipei 112, Taiwan, Republic of China1
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China2
Department of Pathology, Tri-service General Hospital, Taipei, Taiwan, Republic of China3
Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan, Republic of China4
Author for correspondence: Shiau-Ting Hu. Fax +886 2 2821 2880. e-mail tingnahu{at}ym.edu.tw
In dengue virus (DEN) particles, the core protein is a structural protein of the nucleocapsid. The core protein is known to be present in the nucleus of DEN-infected cells but there have been conflicting reports as to whether it is also present in the nucleolus. To clarify this, the intracellular location of the core protein was examined using a monoclonal antibody, 15B11, which was produced in this study. Immunofluorescence staining with this antibody demonstrated that the core protein first appeared in the cytoplasm and then in the nuclei and nucleoli of infected cells. Nuclear localization of the core protein was determined to be independent of other DEN proteins, since recombinant core proteins still entered the nuclei and nucleoli of cells transfected with only the core protein gene. Three putative nuclear localization signal motifs have been predicted to be present on the core protein. Deletion of the first one (KKAR), located at aa 6–9, and mutation of the second one (KKSK), located at aa 73–76, did not eliminate the nuclear localization property of the core protein. The third motif with a bipartite structure, RKeigrmlnilnRRRR, located at aa 85–100, was determined to be responsible for the nuclear localization of the core protein, since the core protein without this motif was located exclusively in the cytoplasm of DEN-infected cells and that this motif mediated nuclear localization of a normally cytoplasmic protein.
This article has been cited by other articles:
|
|
 |
|
  S. Sangiambut, P. Keelapang, J. Aaskov, C. Puttikhunt, W. Kasinrerk, P. Malasit, and N. Sittisombut Multiple regions in dengue virus capsid protein contribute to nuclear localization during virus infection J. Gen. Virol., May 1, 2008; 89(5): 1254 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Mori, T. Yamashita, Y. Tanaka, Y. Tsuda, T. Abe, K. Moriishi, and Y. Matsuura Processing of Capsid Protein by Cathepsin L Plays a Crucial Role in Replication of Japanese Encephalitis Virus in Neural and Macrophage Cells J. Virol., August 15, 2007; 81(16): 8477 - 8487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Melen, L. Kinnunen, R. Fagerlund, N. Ikonen, K. Y. Twu, R. M. Krug, and I. Julkunen Nuclear and Nucleolar Targeting of Influenza A Virus NS1 Protein: Striking Differences between Different Virus Subtypes J. Virol., June 1, 2007; 81(11): 5995 - 6006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Clyde, J. L. Kyle, and E. Harris Recent Advances in Deciphering Viral and Host Determinants of Dengue Virus Replication and Pathogenesis J. Virol., December 1, 2006; 80(23): 11418 - 11431. [Full Text] [PDF]
|
|
|
|
|
|
K. Clyde and E. Harris RNA Secondary Structure in the Coding Region of Dengue Virus Type 2 Directs Translation Start Codon Selection and Is Required for Viral Replication J. Virol., March 1, 2006; 80(5): 2170 - 2182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mori, T. Okabayashi, T. Yamashita, Z. Zhao, T. Wakita, K. Yasui, F. Hasebe, M. Tadano, E. Konishi, K. Moriishi, et al. Nuclear Localization of Japanese Encephalitis Virus Core Protein Enhances Viral Replication J. Virol., March 15, 2005; 79(6): 3448 - 3458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Wang, W.-J. Syu, and S.-T. Hu Identification of the homotypic interaction domain of the core protein of dengue virus type 2 J. Gen. Virol., August 1, 2004; 85(8): 2307 - 2314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Fielding, Y.-J. Tan, S. Shuo, T. H. P. Tan, E.-E. Ooi, S. G. Lim, W. Hong, and P.-Y. Goh Characterization of a Unique Group-Specific Protein (U122) of the Severe Acute Respiratory Syndrome Coronavirus J. Virol., July 15, 2004; 78(14): 7311 - 7318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sabherwal, K. U. Schneider, R. J. Blaschke, A. Marchini, and G. Rappold Impairment of SHOX nuclear localization as a cause for Leri-Weill syndrome J. Cell Sci., June 15, 2004; 117(14): 3041 - 3048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Pesavento, N. J. MacLachlan, L. Dillard-Telm, C. K. Grant, and K. F. Hurley Pathologic, Immunohistochemical, and Electron Microscopic Findings in Naturally Occurring Virulent Systemic Feline Calicivirus Infection in Cats Vet. Pathol., May 1, 2004; 41(3): 257 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ma, C. T. Jones, T. D. Groesch, R. J. Kuhn, and C. B. Post Solution structure of dengue virus capsid protein reveals another fold PNAS, March 9, 2004; 101(10): 3414 - 3419. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
