HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Assis Filho, F. M. Articles by Deom, C. M. Search for Related Content PubMed PubMed Citation Articles by de Assis Filho, F. M. Articles by Deom, C. M. Agricola Articles by de Assis Filho, F. M. Articles by Deom, C. M.

Symptom induction by Cowpea chlorotic mottle virus on Vigna unguiculata is determined by amino acid residue 151 in the coat protein

Department of Plant Pathology, The University of Georgia, Athens, GA 30602, USA¹

Author for correspondence: Carl Deom. Fax +1 706 542 1262. e-mail deom{at}uga.edu

The type strain of Cowpea chlorotic mottle virus (CCMV-T) produces a bright chlorosis in cowpea (Vigna unguiculata cv. California Blackeye). The attenuated variant (CCMV-M) induces mild green mottle symptoms that were previously mapped to RNA 3. Restriction fragment exchanges between RNA 3 cDNA clones of CCMV-T and CCMV-M that generate infectious transcripts and site-directed mutagenesis indicated that the codon encoding amino acid residue 151 of the coat protein determines the symptom phenotypes of CCMV-T and CCMV-M. Amino acid 151 is within an -helical structure required for calcium ion binding and virus particle stability. No differences in virion stability or accumulation were detected between CCMV-T and CCMV-M. Mutational analysis suggested that the amino acid at position 151 and not the nucleotide sequence induce the symptom phenotype. Thus, it is likely that subtle influences by amino acid residue 151 in coat protein–host interactions result in chlorotic and mild green mottle symptoms.

This article has been cited by other articles:

				H. Hirata, X. Lu, Y. Yamaji, S. Kagiwada, M. Ugaki, and S. Namba A single silent substitution in the genome of Apple stem grooving virus causes symptom attenuation J. Gen. Virol., September 1, 2003; 84(9): 2579 - 2583. [Abstract] [Full Text] [PDF]