HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bichr, S. Articles by Bréchot, C. Search for Related Content PubMed PubMed Citation Articles by Bichr, S. Articles by Bréchot, C. Agricola Articles by Bichr, S. Articles by Bréchot, C.

Detection of neutralizing antibodies to hepatitis C virus using a biliary cell infection model

Inserm U370, Faculté de Médecine Necker, 156 rue de Vaugirard, 75730 Paris Cédex 15, France¹
Inserm U25; Hôpital Necker; 75015 Paris, France²
Innogenetics, Gent, Belgium³

Author for correspondence: Christian Bréchot. Fax +33 1 40615581. e-mail Brechot{at}necker.fr

The identification and characterization of neutralizing anti-hepatitis C virus (HCV) antibodies may have a major impact on understanding HCV pathogenesis. However, to date, their detection has only been based on the inhibition of either the E2 envelope protein or HCV virions binding to different target cells. The permissivity of primary biliary cells for HCV infection has been demonstrated previously. In the present report, infection of biliary cells was demonstrated further by combining PCR and immunohistochemical detection of the HCV core protein. This study demonstrates, using both serum and purified IgG, the presence of neutralizing anti-HCV antibodies in the serum of patients showing long-term response to antiviral therapy. Overall, the usefulness of the primary biliary cell infection model to investigate anti-HCV neutralization is shown.

This article has been cited by other articles:

				D. Steinmann, H. Barth, B. Gissler, P. Schurmann, M. I. Adah, J. T. Gerlach, G. R. Pape, E. Depla, D. Jacobs, G. Maertens, et al. Inhibition of Hepatitis C Virus-Like Particle Binding to Target Cells by Antiviral Antibodies in Acute and Chronic Hepatitis C J. Virol., September 1, 2004; 78(17): 9030 - 9040. [Abstract] [Full Text] [PDF]