HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jones, L. R. Articles by Weber, E. L. Search for Related Content PubMed PubMed Citation Articles by Jones, L. R. Articles by Weber, E. L. Agricola Articles by Jones, L. R. Articles by Weber, E. L.

Quasispecies in the 5' untranslated genomic region of bovine viral diarrhoea virus from a single individual

Instituto de Virología, CICVyA, INTA-Castelar, CC77 (1708) Morón, Buenos Aires, Argentina¹
Instituto de Microbiología y Zoología Agrícola, CICVyA, INTA-Castelar, CC77 (1708) Morón, Buenos Aires, Argentina²
Consejo Nacional de Investigaciones Científicas, Argentina³

Author for correspondence: Leandro Jones. Fax +54 11 4621 1743. e-mail ljones{at}cicv.inta.gov.ar

The variability of the 5' untranslated genomic region (5'UTR) of bovine viral diarrhoea virus (BVDV) RNA obtained from a single individual was analysed. Lung, kidney and spleen tissues from a naturally infected foetus were used as the source of viral RNA. A fragment of 288 bases of the internal ribosome entry site from the BVDV 5'UTR was amplified by RT–PCR using a proofreading DNA polymerase. PCR products were cloned into pGem and, subsequently, transformed into Escherichia coli. The single-strand conformational polymorphisms of 158 lung-derived clones were analysed; a total of 11 banding patterns was observed. DNAs corresponding to all patterns were sequenced. Of the randomly selected clones, 11 and 10 clones derived from the kidney and spleen, respectively, were also sequenced. All sequences presented differences ranging from 1 to 6 nt substitutions. Analysis of the secondary structure of the variant sequences and comparisons to variant nucleotide sites from the 5'UTR of several BVDV isolates showed that the observed changes were almost free of randomness. Clustering and phylogenetic analyses suggested the existence of low-kinetic variants. BVDV quasispecies may be involved in establishing persistent infections by means of eluding maternal antibodies. The methods described here may be adapted easily both to analyse large numbers of samples from other genomic regions and for the study of BVDV quasispecies evolution in other systems.

This article has been cited by other articles:

				Y. Sasaki, A. Kai, Y. Hayashi, T. Shinkai, Y. Noguchi, M. Hasegawa, K. Sadamasu, K. Mori, Y. Tabei, M. Nagashima, et al. Multiple Viral Infections and Genomic Divergence among Noroviruses during an Outbreak of Acute Gastroenteritis. J. Clin. Microbiol., March 1, 2006; 44(3): 790 - 797. [Abstract] [Full Text] [PDF]

				G. Jerzak, K. A. Bernard, L. D. Kramer, and G. D. Ebel Genetic variation in West Nile virus from naturally infected mosquitoes and birds suggests quasispecies structure and strong purifying selection J. Gen. Virol., August 1, 2005; 86(8): 2175 - 2183. [Abstract] [Full Text] [PDF]