| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Wohl Virion Centre, The Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1T 4JF, UK1
Author for correspondence: Yasuhiro Takeuchi. Fax +44 207 679 9555. e-mail y.takeuchi{at}ucl.ac.uk
Two pig endogenous retroviruses (PERV), PERV-A and -B, productively infect human cells and are therefore considered to constitute a potential risk in pig-to-human xenotransplantation. A PCR-based cloning technique to isolate infectious PERV proviruses was established. Overlapping 3' half and 5' halves of PERV proviral genomes were amplified using DNA extracted from human 293 cells infected with PERV-A or -B. These clones were fused at a unique restriction site in the overlapping region and tested for their infectivity. Representative constructs possessed the same infectious properties as their parent isolates. We also developed a polyclonal anti-PERV serum by using recombinant PERV capsid protein derived from one of the infectious constructs as immunogen and established an immunocytological method for detection and titration of PERV infection. This detection method proved to be more sensitive than the current method of choice (transfer of MLV-lacZ vectors) for infectivity assessment of PERV. These findings should be considered for future characterization of PERV isolates.
This article has been cited by other articles:
|
|
 |
|
  A. Wood, B. L. J. Webb, B. Bartosch, T. Schaller, Y. Takeuchi, and G. J. Towers Porcine endogenous retroviruses PERV A and A/C recombinant are insensitive to a range of divergent mammalian TRIM5{alpha} proteins including human TRIM5{alpha} J. Gen. Virol., March 1, 2009; 90(3): 702 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Moalic, H. Felix, Y. Takeuchi, A. Jestin, and Y. Blanchard Genome Areas with High Gene Density and CpG Island Neighborhood Strongly Attract Porcine Endogenous Retrovirus for Integration and Favor the Formation of Hot Spots J. Virol., February 15, 2009; 83(4): 1920 - 1929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takeuchi, M. O. McClure, and M. Pizzato Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research J. Virol., December 15, 2008; 82(24): 12585 - 12588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Dong, S. Kim, S. Hong, J. Das Gupta, K. Malathi, E. A. Klein, D. Ganem, J. L. DeRisi, S. A. Chow, and R. H. Silverman From the Cover: An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors PNAS, January 30, 2007; 104(5): 1655 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Harrison, Y. Takeuchi, B. Bartosch, and J. P. Stoye Determinants of High Titer in Recombinant Porcine Endogenous Retroviruses J. Virol., December 15, 2004; 78(24): 13871 - 13879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bartosch, D. Stefanidis, R. Myers, R. Weiss, C. Patience, and Y. Takeuchi Evidence and Consequence of Porcine Endogenous Retrovirus Recombination J. Virol., December 15, 2004; 78(24): 13880 - 13890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Scobie, S. Taylor, N. A. Logan, S. Meikle, D. Onions, C. Patience, and G. Quinn Characterization of germline porcine endogenous retroviruses from Large White pig J. Gen. Virol., August 1, 2004; 85(8): 2421 - 2428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Magre, Y. Takeuchi, G. Langford, A. Richards, C. Patience, and R. Weiss Reduced Sensitivity to Human Serum Inactivation of Enveloped Viruses Produced by Pig Cells Transgenic for Human CD55 or Deficient for the Galactosyl-{alpha}(1-3) Galactosyl Epitope J. Virol., June 1, 2004; 78(11): 5812 - 5819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Scobie, S. Taylor, J. C. Wood, K. M. Suling, G. Quinn, S. Meikle, C. Patience, H.-J. Schuurman, and D. E. Onions Absence of Replication-Competent Human-Tropic Porcine Endogenous Retroviruses in the Germ Line DNA of Inbred Miniature Swine J. Virol., March 1, 2004; 78(5): 2502 - 2509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Tonjes and M. Niebert Relative Age of Proviral Porcine Endogenous Retrovirus Sequences in Sus scrofa Based on the Molecular Clock Hypothesis J. Virol., November 15, 2003; 77(22): 12363 - 12368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Klymiuk, M. Muller, G. Brem, and B. Aigner Recombination analysis of human-tropic porcine endogenous retroviruses J. Gen. Virol., October 1, 2003; 84(10): 2729 - 2734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. V. Del Priore, O. Ishida, E. W. Johnson, Y. Sheng, D. B. Jacoby, L. Geng, T. H. Tezel, and H. J. Kaplan Triple Immune Suppression Increases Short-Term Survival of Porcine Fetal Retinal Pigment Epithelium Xenografts Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 4044 - 4053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Ericsson, Y. Takeuchi, C. Templin, G. Quinn, S. F. Farhadian, J. C. Wood, B. A. Oldmixon, K. M. Suling, J. K. Ishii, Y. Kitagawa, et al. Identification of receptors for pig endogenous retrovirus PNAS, May 27, 2003; 100(11): 6759 - 6764. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
