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Department of Medicine, University of Cambridge, PO Box 157, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK
Correspondence
John Sinclair
js{at}mole.bio.cam.ac.uk
The repression of human cytomegalovirus immediate-early (IE) lytic gene expression is crucial for the maintenance of the latent viral state. By using conditionally permissive cell lines, which provide a good model for the differentiation state-dependent repression of IE gene expression, we have identified several cellular factors that bind to the major immediate-early promoter (MIEP) and whose expression is down-regulated after differentiation to a permissive phenotype. Here we show that the cellular protein Ets-2 Repressor Factor (ERF) physically interacts with the MIEP and represses MIEP activity in undifferentiated non-permissive T2 embryonal carcinoma cells. This factor binds to the dyad element and the 21 bp repeats within the MIEP – regions known to be important for the negative regulation of MIEP activity. Finally, we show that following differentiation to a permissive phenotype ERF's repressive effects are severely abrogated.
Present address: Infectious Diseases Clinical Research Unit, The Lung Institute, University of Cape Town, Cape Town, South Africa
This article has been cited by other articles:
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J. Sinclair and P. Sissons Latency and reactivation of human cytomegalovirus J. Gen. Virol., July 1, 2006; 87(7): 1763 - 1779. [Abstract] [Full Text] [PDF]
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M. B. Reeves, P. J. Lehner, J. G. P. Sissons, and J. H. Sinclair An in vitro model for the regulation of human cytomegalovirus latency and reactivation in dendritic cells by chromatin remodelling J. Gen. Virol., November 1, 2005; 86(11): 2949 - 2954. [Abstract] [Full Text] [PDF]
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 M. B. Reeves, P. A. MacAry, P. J. Lehner, J. G. P. Sissons, and J. H. Sinclair Latency, chromatin remodeling, and reactivation of human cytomegalovirus in the dendritic cells of healthy carriers PNAS, March 15, 2005; 102(11): 4140 - 4145. [Abstract] [Full Text] [PDF]
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E. Wright, M. Bain, L. Teague, J. Murphy, and J. Sinclair Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells J. Gen. Virol., March 1, 2005; 86(3): 535 - 544. [Abstract] [Full Text] [PDF]
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 A. L. McNees, J. A. Mahr, D. Ornelles, and L. R. Gooding Postinternalization Inhibition of Adenovirus Gene Expression and Infectious Virus Production in Human T-Cell Lines J. Virol., July 1, 2004; 78(13): 6955 - 6966. [Abstract] [Full Text] [PDF]
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