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1 Laboratory for Clinical and Molecular Virology, The University of Edinburgh, Summerhall Square, Edinburgh EH9 1QH, UK
2 Scottish Centre for Genomic Technology and Informatics, The University of Edinburgh, Summerhall Square, Edinburgh EH9 1QH, UK
3 MRC Centre for Inflammation Research, The University of Edinburgh, Summerhall Square, Edinburgh EH9 1QH, UK
Correspondence
Bahram Ebrahimi
ebrahimi{at}staffmail.ed.ac.uk
The murine gammaherpesvirus-68 genome encodes 73 protein-coding open reading frames with extensive similarities to human 
2 herpesviruses, as well as unique genes and cellular homologues. We performed transcriptome analysis of stage-specific viral RNA during permissive infection using an oligonucleotide-based microarray. Using this approach, M4, K3, ORF38, ORF50, ORF57 and ORF73 were designated as immediate-early genes based on cycloheximide treatment. The microarray analysis also identified 10 transcripts with early expression kinetics, 32 transcripts with early-late expression kinetics and 29 transcripts with late expression kinetics. The latter group consisted mainly of structural proteins, and showed high expression levels relative to other viral transcripts. Moreover, we detected all eight tRNA-like transcripts in the presence of cycloheximide and phosphonoacetic acid. Lytic infection with MHV-68 also resulted in a significant reduction in the expression of cellular transcripts included in the DNA chip. This global approach to viral transcript analysis offers a powerful system for examining molecular transitions between lytic and latent virus infections associated with disease pathogenesis.
Present address: Departamento de Bioquimica y Biología Molecular, Facultad de Medicina, 02071 Albacete, Spain.
Present address: Department of Medical Microbiology, The University of Liverpool, Liverpool L69 3GA, UK.
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