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Identification of cis-acting sequences required for selective packaging of bovine adenovirus type 3 DNA

Linong Zhang

Vaccine and Infectious Disease Organization, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3

Correspondence
Suresh Kumar Tikoo
Tikoo{at}Sask.Usask.CA

The assembly of adenovirus particles is a multistep process, in which viral genomic DNA is selected and subsequently inserted into preformed empty capsids. The selective encapsidation of the adenovirus genome is directed by cis-acting packaging motifs, termed A repeats due to their AT-rich character in DNA sequence. A repeats are usually located at the left end of the viral genome. In this report, the construction and analysis of bovine adenovirus type 3 (BAdV-3) mutants containing deletion mutations introduced into the AT-rich regions are described. The main cis-acting packaging domains of BAdV-3 were localized between nt 224 and 540 relative to the left end of the viral genome. They displayed a functional redundancy and followed a hierarchy of importance. In addition, the results demonstrated that not all of the AT-rich units functioned as cis-acting packaging motifs.

Present address: Aventis Pasteur Limited, Toronto, Canada.

This article has been cited by other articles:

				L. Xing and S. K. Tikoo E1A promoter of bovine adenovirus type 3 J. Gen. Virol., December 1, 2006; 87(12): 3539 - 3544. [Abstract] [Full Text] [PDF]