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Transcript mapping of the ‘early’ genes of Orf virus

Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK

Correspondence
Colin McInnes
mcinc{at}mri.sari.ac.uk

The full complement of genes encoded by Orf virus (ORFV) is not yet known. A cDNA library was constructed using mRNA isolated 5 h post-infection from cells infected with ORFV in vitro and grown in the presence of cytosine arabinoside. Using 12 non-overlapping probes representing the entire genome of the Orf-11 strain of the virus, cDNA clones representing individual genes expressed early in infection were isolated. Thirty-eight early genes were identified, either via isolation of their cDNA from the library or via Northern blotting. Twenty-nine of the isolated cDNAs represented orthologues of other poxvirus genes or had been identified previously as genes of ORFV, whilst seven appeared unrelated to any known poxvirus gene or indeed to any known gene in the DNA databases. The sequences described in this paper constitute approximately 30 kb of the ORFV genome and contain the complete or partial sequence of 47 genes.

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