| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS et Université Aix-Marseille I et II, ESIL, Campus de Luminy, 13288 Marseille Cedex 09, France
Correspondence
Sonia Longhi
longhi{at}afmb.cnrs-mrs.fr
The existence and extent of disorder within the replicative complex (N, P and the polymerase, L) of Paramyxovirinae were investigated, drawing on the discovery that the N-terminal moiety of the phosphoprotein (P) and the C-terminal moiety of the nucleoprotein (N) of measles virus are intrinsically unstructured. We show that intrinsic disorder is a widespread property within Paramyxovirinae N and P, using a combination of different computational approaches relying on different physico-chemical concepts. Notably, experimental support that has often gone unnoticed for most of the predictions has been found in the literature. Identification of disordered regions allows the unveiling of a common organization in all Paramyxovirinae P, which are composed of six modules defined on the basis of structure or sequence conservation. The possible functional significance of intrinsic disorder is discussed in the light of experimental data, which show that unstructured regions of P and N are involved in numerous interactions with several protein and protein–RNA partners. This study provides a contribution to the rather poorly investigated field of intrinsically disordered proteins and helps in targeting protein domains for structural studies.
Present address: Ecole de l'ADN, Association Grand Luminy, Case 922, Bat. CCIMP, 13288 Marseille Cedex 09, France.
This article has been cited by other articles:
|
|
 |
|
  C.-K. Chang, Y.-L. Hsu, Y.-H. Chang, F.-A. Chao, M.-C. Wu, Y.-S. Huang, C.-K. Hu, and T.-H. Huang Multiple Nucleic Acid Binding Sites and Intrinsic Disorder of Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein: Implications for Ribonucleocapsid Protein Packaging J. Virol., March 1, 2009; 83(5): 2255 - 2264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. John, T. Wang, S. Steffen, S. Longhi, C. S. Schmaljohn, and C. B. Jonsson Ebola Virus VP30 Is an RNA Binding Protein J. Virol., September 1, 2007; 81(17): 8967 - 8976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. White, J.-J. Yoon, J. K. Lee, A. Sun, Y. Du, H. Fu, J. P. Snyder, and R. K. Plemper Nonnucleoside Inhibitor of Measles Virus RNA-Dependent RNA Polymerase Complex Activity Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2293 - 2303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Houben, D. Marion, N. Tarbouriech, R. W. H. Ruigrok, and L. Blanchard Interaction of the C-Terminal Domains of Sendai Virus N and P Proteins: Comparison of Polymerase-Nucleocapsid Interactions within the Paramyxovirus Family J. Virol., July 1, 2007; 81(13): 6807 - 6816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Cowton, D. R. McGivern, and R. Fearns Unravelling the complexities of respiratory syncytial virus RNA synthesis J. Gen. Virol., July 1, 2006; 87(7): 1805 - 1821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Llorente, B. Garcia-Barreno, M. Calero, E. Camafeita, J. A. Lopez, S. Longhi, F. Ferron, P. F. Varela, and J. A. Melero Structural analysis of the human respiratory syncytial virus phosphoprotein: characterization of an {alpha}-helical domain involved in oligomerization J. Gen. Virol., January 1, 2006; 87(1): 159 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chen, J.-C. Cortay, I. R. Logan, V. Sapountzi, C. N. Robson, and D. Gerlier Inhibition of Ubiquitination and Stabilization of Human Ubiquitin E3 Ligase PIRH2 by Measles Virus Phosphoprotein J. Virol., September 15, 2005; 79(18): 11824 - 11836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Laine, J. M. Bourhis, S. Longhi, M. Flacher, L. Cassard, B. Canard, C. Sautes-Fridman, C. Rabourdin-Combe, and H. Valentin Measles virus nucleoprotein induces cell-proliferation arrest and apoptosis through NTAIL-NR and NCORE-Fc{gamma}RIIB1 interactions, respectively J. Gen. Virol., June 1, 2005; 86(6): 1771 - 1784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Brown, F. M. Collins, W. P. Duprex, M. D. Baron, T. Barrett, and B. K. Rima 'Rescue' of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins J. Gen. Virol., April 1, 2005; 86(4): 1077 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ferron, C. Rancurel, S. Longhi, C. Cambillau, B. Henrissat, and B. Canard VaZyMolO: a tool to define and classify modularity in viral proteins J. Gen. Virol., March 1, 2005; 86(3): 743 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Devaux and R. Cattaneo Measles Virus Phosphoprotein Gene Products: Conformational Flexibility of the P/V Protein Amino-Terminal Domain and C Protein Infectivity Factor Function J. Virol., November 1, 2004; 78(21): 11632 - 11640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Kingston, D. J. Hamel, L. S. Gay, F. W. Dahlquist, and B. W. Matthews Structural basis for the attachment of a paramyxoviral polymerase to its template PNAS, June 1, 2004; 101(22): 8301 - 8306. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
