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Short Communication |
1 Center for Cancer and Immunology Research, Children's Research Institute, Children's National Medical Center, George Washington University School of Medicine and Health Sciences, 111 Michigan Avenue, NW, Washington, DC 20010, USA
2 Department of Pediatrics, George Washington University School of Medicine and Health Sciences, 111 Michigan Avenue, NW, Washington, DC 20010, USA
Correspondence
Anamaris M. Colberg-Poley
acolberg-poley{at}cnmcresearch.org
Differentially processed human cytomegalovirus (HCMV) UL37 RNAs encode biologically significant proteins. Due to the recent discovery of alternative UL37 exon 3 (UL37x3) splice donors, permissively infected cells were thoroughly examined for additional alternatively spliced UL37 RNAs. Newly described donors within UL37 exon 1 (nt 52520) and intron 1 (nt 52209) as well as UL37x3 di (nt 50770) and dii (nt 50782) were differentially spliced to known downstream UL37 acceptors. The alternatively spliced UL37S, UL37L, UL37di and UL37dii RNAs predictably encode proteins of 83, 163, 217 and 213 residues, respectively, which share UL37x1 N-terminal sequences but differ downstream in their C termini. Moreover, temporal expression of the alternatively spliced UL37 RNAs differs during HCMV infection. The complexity of UL37 pre-mRNA processing is evidenced by the detection of 11 UL37 spliced and unspliced UL37x1 RNAs in HCMV-infected cells. Based upon these data, a revised HCMV UL37 gene map is presented, which incorporates all RNA species detected during permissive infection.
This article has been cited by other articles:
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  P. Bozidis, C. D. Williamson, and A. M. Colberg-Poley Mitochondrial and Secretory Human Cytomegalovirus UL37 Proteins Traffic into Mitochondrion-Associated Membranes of Human Cells J. Virol., March 15, 2008; 82(6): 2715 - 2726. [Abstract] [Full Text] [PDF]
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S. Terhune, E. Torigoi, N. Moorman, M. Silva, Z. Qian, T. Shenk, and D. Yu Human Cytomegalovirus UL38 Protein Blocks Apoptosis J. Virol., April 1, 2007; 81(7): 3109 - 3123. [Abstract] [Full Text] [PDF]
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 R. Sharon-Friling, J. Goodhouse, A. M. Colberg-Poley, and T. Shenk Human cytomegalovirus pUL37x1 induces the release of endoplasmic reticulum calcium stores PNAS, December 12, 2006; 103(50): 19117 - 19122. [Abstract] [Full Text] [PDF]
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M. S. Mavinakere, C. D. Williamson, V. S. Goldmacher, and A. M. Colberg-Poley Processing of Human Cytomegalovirus UL37 Mutant Glycoproteins in the Endoplasmic Reticulum Lumen prior to Mitochondrial Importation J. Virol., July 15, 2006; 80(14): 6771 - 6783. [Abstract] [Full Text] [PDF]
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S. Tamrakar, A. J. Kapasi, and D. H. Spector Human Cytomegalovirus Infection Induces Specific Hyperphosphorylation of the Carboxyl-Terminal Domain of the Large Subunit of RNA Polymerase II That Is Associated with Changes in the Abundance, Activity, and Localization of cdk9 and cdk7 J. Virol., December 15, 2005; 79(24): 15477 - 15493. [Abstract] [Full Text] [PDF]
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 R. Adair, G. W. Liebisch, Y. Su, and A. M. Colberg-Poley Alteration of cellular RNA splicing and polyadenylation machineries during productive human cytomegalovirus infection J. Gen. Virol., December 1, 2004; 85(12): 3541 - 3553. [Abstract] [Full Text] [PDF]
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M. Reboredo, R. F. Greaves, and G. Hahn Human cytomegalovirus proteins encoded by UL37 exon 1 protect infected fibroblasts against virus-induced apoptosis and are required for efficient virus replication J. Gen. Virol., December 1, 2004; 85(12): 3555 - 3567. [Abstract] [Full Text] [PDF]
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M. S. Mavinakere and A. M. Colberg-Poley Internal cleavage of the human cytomegalovirus UL37 immediate-early glycoprotein and divergent trafficking of its proteolytic fragments J. Gen. Virol., July 1, 2004; 85(7): 1989 - 1994. [Abstract] [Full Text] [PDF]
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M. S. Mavinakere and A. M. Colberg-Poley Dual targeting of the human cytomegalovirus UL37 exon 1 protein during permissive infection J. Gen. Virol., February 1, 2004; 85(2): 323 - 329. [Abstract] [Full Text] [PDF]
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