| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Short Communication |
United States Department of Agriculture, Agricultural Research Service, 1102 S. Goodwin Avenue, University of Illinois at Urbana/Champaign, Urbana, IL 61801, USA
Correspondence
Leslie Domier
l-domier{at}uiuc.edu
The RNA genome of Rhopalosiphum padi virus (RhPV), like other members of the Dicistroviridae, contains two open reading frames that are preceded by internal ribosome entry sites (IRESs). To compare the activities of the two RhPV IRESs in insect cells, a system was established for the in vivo transcription and translation of plasmid templates containing the IRESs. In this system, the two RhPV IRESs directed initiation of translation from bicistronic plasmids with equal efficiency. Competition was observed between the two IRESs when they were in cis in a bicistronic plasmid. A mutation that disrupted the 3'-proximal pseudoknot of the intergenic (IG) IRES reduced translation initiation in vivo. Similarly, mutations in the RhPV IG IRES disrupted its ability to bind 80S particles in vitro. The two IRESs preferentially labelled proteins of different masses in UV cross-linking experiments, illustrating the different translation initiation mechanisms employed by the two elements.
This article has been cited by other articles:
|
|
 |
|
  E. Maori, S. Lavi, R. Mozes-Koch, Y. Gantman, Y. Peretz, O. Edelbaum, E. Tanne, and I. Sela Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination J. Gen. Virol., December 1, 2007; 88(12): 3428 - 3438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lu, Y. Hu, L. Hu, S. Zong, D. Cai, J. Wang, H. Yu, and J. Zhang Ectropis obliqua picorna-like virus IRES-driven internal initiation of translation in cell systems derived from different origins J. Gen. Virol., October 1, 2007; 88(10): 2834 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Ongus, E. C. Roode, C. W. A. Pleij, J. M. Vlak, and M. M. van Oers The 5' non-translated region of Varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells. J. Gen. Virol., November 1, 2006; 87(Pt 11): 3397 - 3407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. de Miranda, M. Drebot, S. Tyler, M. Shen, C. E. Cameron, D. B. Stoltz, and S. M. Camazine Complete nucleotide sequence of Kashmir bee virus and comparison with acute bee paralysis virus J. Gen. Virol., August 1, 2004; 85(8): 2263 - 2270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Royall, K. E. Woolaway, J. Schacherl, S. Kubick, G. J. Belsham, and L. O. Roberts The Rhopalosiphum padi virus 5' internal ribosome entry site is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates: implications for the baculovirus expression system J. Gen. Virol., June 1, 2004; 85(6): 1565 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Shen and W. A. Miller The 3' Untranslated Region of Tobacco Necrosis Virus RNA Contains a Barley Yellow Dwarf Virus-Like Cap-Independent Translation Element J. Virol., May 1, 2004; 78(9): 4655 - 4664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shibuya, T. Nishiyama, Y. Kanamori, H. Saito, and N. Nakashima Conditional Rather than Absolute Requirements of the Capsid Coding Sequence for Initiation of Methionine-Independent Translation in Plautia stali Intestine Virus J. Virol., November 15, 2003; 77(22): 12002 - 12010. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
