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Induction of apoptosis in an insect cell line, IPLB-Ld652Y, infected with nucleopolyhedroviruses

Kenichi Yanagimoto^1,

Barbara A. Laviña-Caoili^1,

¹ Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
² Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan

Correspondence
Michihiro Kobayashi
michihir{at}agr.nagoya-u.ac.jp

Ld652Y cells derived from the gypsy moth, Lymantria dispar, were infected with seven different nucleopolyhedroviruses (NPVs) including those from Autographa californica, Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Spodoptera exigua (SeMNPV), L. dispar, Orgyia pseudotsugata (OpMNPV) and Spodoptera litura (SpltMNPV). The results showed that Ld652Y cells infected with BmNPV, HycuNPV, SeMNPV, OpMNPV and SpltMNPV underwent apoptosis, displaying apoptotic bodies, characteristic DNA fragmentation and increased caspase-3-like protease activity; HycuNPV induced the most severe apoptosis. In HycuNPV-infected Ld652Y cells, a considerable amount of viral DNA was synthesized although there was no detectable yield of budded virions and polyhedrin. Northern blot and immunoblot analyses revealed that HycuNPV inhibitor of apoptosis 3 (IAP3), which has been shown to function in Sf9 cells, was expressed in HycuNPV-infected Ld652Y cells at a level higher than or comparable with that in HycuNPV-infected SpIm cells, which produced a high titre of progeny virions without any apoptotic response. These results imply that the relative ease of apoptosis induction in NPV-infected Ld652Y cells is largely dependent on inherent cellular properties rather than functions of the respective NPVs, and indicate that the defect in progeny virion production is not merely due to the virus-induced apoptosis in HycuNPV-infected Ld652Y cells.

Present address: Nikken Foods Co., Ltd, Haruoka 723-1, Hukuroi, Shizuoka 437-0122, Japan.

Present address: Insect Pathology Laboratory, Department of Entomology, College of Agriculture, University of the Philippines Los Baños College, Laguna 4031, Philippines.

This article has been cited by other articles:

				M. Ikeda, M. Shikata, N. Shirata, S. Chaeychomsri, and M. Kobayashi Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome J. Gen. Virol., September 1, 2006; 87(9): 2549 - 2562. [Abstract] [Full Text] [PDF]

				H. Ishikawa, M. Ikeda, C. A. F. Alves, S. M. Thiem, and M. Kobayashi Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells Derived from L. dispar J. Virol., November 15, 2004; 78(22): 12703 - 12708. [Abstract] [Full Text] [PDF]