HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kohl, A. Articles by Elliott, R. M. Search for Related Content PubMed PubMed Citation Articles by Kohl, A. Articles by Elliott, R. M. Agricola Articles by Kohl, A. Articles by Elliott, R. M.

Effects of a point mutation in the 3' end of the S genome segment of naturally occurring and engineered Bunyamwera viruses

Anne Bridgen^1,

Ewan Dunn^1,

¹ Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK
² Department of Microbiology, University of Alabama School of Medicine, 845 19th Street South, Birmingham, AL 35294, USA

Correspondence
Richard Elliott
r.elliott{at}vir.gla.ac.uk

The genome of Bunyamwera virus (BUN) consists of three segments of single-stranded RNA of negative polarity. The smallest segment, S, encodes the N protein and a nonstructural protein called NSs. We recently described a mutant virus (BUNdelNSs) that does not express NSs but overexpresses N and grows to lower titres than wild-type (wt) BUN. Here we report a BUNdelNSs variant that expresses lower levels of N protein and grows to higher titres. Sequencing of the 3' and 5' termini of the BUNdelNSs S RNA segment and analysis using a minireplicon system show that the N overexpressing phenotype results from a single nucleotide substitution at position 16 in the 3' terminus. This mutation could also be detected in wtBUN populations, and was isolated by plaquing a ‘wt’ variant carrying the mutation. This variant was found to express increased N and NSs levels, and grew to lower titres than wtBUN.

Present address: Department of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, UK.

Present address: Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Hanover, New Hampshire 03755, USA.

This article has been cited by other articles:

				G. Blakqori and F. Weber Efficient cDNA-Based Rescue of La Crosse Bunyaviruses Expressing or Lacking the Nonstructural Protein NSs J. Virol., August 15, 2005; 79(16): 10420 - 10428. [Abstract] [Full Text] [PDF]

				A. C. Lowen, A. Boyd, J. K. Fazakerley, and R. M. Elliott Attenuation of Bunyavirus Replication by Rearrangement of Viral Coding and Noncoding Sequences J. Virol., June 1, 2005; 79(11): 6940 - 6946. [Abstract] [Full Text] [PDF]

				A. Kohl, E. F. Dunn, A. C. Lowen, and R. M. Elliott Complementarity, sequence and structural elements within the 3' and 5' non-coding regions of the Bunyamwera orthobunyavirus S segment determine promoter strength J. Gen. Virol., November 1, 2004; 85(11): 3269 - 3278. [Abstract] [Full Text] [PDF]

				A. Kohl, T. J. Hart, C. Noonan, E. Royall, L. O. Roberts, and R. M. Elliott A Bunyamwera Virus Minireplicon System in Mosquito Cells J. Virol., June 1, 2004; 78(11): 5679 - 5685. [Abstract] [Full Text] [PDF]