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J Gen Virol 84 (2003), 1281-1285; DOI 10.1099/vir.0.18998-0

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© 2003 Society for General Microbiology

Short Communication

Construction and characterization of pentacistronic retrovirus vectors

Pablo de Felipe{dagger} and Marta Izquierdo

Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain

Correspondence
Marta Izquierdo
mizquierdo{at}cbm.uam.es

The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size ~7·9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.

{dagger}Present address: Centre for Biomolecular Sciences, North Haugh, University of St Andrews, St Andrews, Fife KY16 9ST, UK.




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