J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


J Gen Virol 84 (2003), 1327-1338; DOI 10.1099/vir.0.18874-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heggebø, R.
Right arrow Articles by Jeffrey, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heggebø, R.
Right arrow Articles by Jeffrey, M.
Agricola
Right arrow Articles by Heggebø, R.
Right arrow Articles by Jeffrey, M.
© 2003 Society for General Microbiology

Disease-associated PrP in the enteric nervous system of scrapie-affected Suffolk sheep

Ragna Heggebø1, Lorenzo González2, Charles McL. Press1, Gjermund Gunnes1, Arild Espenes1 and Martin Jeffrey2

1 Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo, Norway
2 Lasswade Veterinary Laboratory, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK

Correspondence
Charles Press
Charles.Press{at}veths.no

Disease-associated prion protein (PrPd) in the enteric nervous system (ENS) of 20- to 24-month-old Suffolk sheep in the late subclinical and early clinical phase of scrapie was studied. Sites in the alimentary tract extending from the forestomachs and abomasum to the colon from scrapie-affected sheep (PrPARQ/ARQ) and scrapie-resistant sheep (PrPARR/ARQ and PrPARR/ARR) were examined. PrPd was found only in scrapie-affected sheep and was most prominent in the ENS when abundant deposits of PrPd were also present in adjacent lymphoid nodules. Immunolabelling with the nerve fibre markers PgP 9.5 and neuron-specific enolase and the satellite cell marker glial fibrillary acidic protein revealed the extensive ganglionated networks of the myenteric and submucosal plexi. Fewer nerve fibres were present in the lamina propria, T-cell dominated interfollicular areas and dome regions of Peyer's patches. A substantial network of nerve fibres was detected in many lymphoid nodules of both the scrapie-affected and scrapie-resistant sheep. Nerve fibres were also detected within the capsule of lymphoid nodules. Electron microscopy revealed the presence of nerves in the lymphoid nodules, showing a close association with follicular dendritic cells, lymphocytes and tingible body macrophages. In demonstrating that lymphoid nodules in the Peyer's patches of scrapie-affected sheep possess a substantial network of nerve fibres, the present study shows that nodules provide close contact between nerve fibres and cell populations known to contain abundant PrPd, including follicular dendritic cells and tingible body macrophages, and that gut-associated lymphoid nodules in sheep may represent an important site for neuroinvasion.

Published ahead of print on 10 February 2003 as DOI 10.1099/vir.0.18874-0.




This article has been cited by other articles:


Home page
Vet PatholHome page
G. McGovern, S. Martin, L. Gonzalez, J. Witz, and M. Jeffrey
Frequency and Distribution of Nerves in Scrapie-affected and Unaffected Peyer's Patches and Lymph Nodes
Vet. Pathol., March 1, 2009; 46(2): 233 - 240.
[Abstract] [Full Text] [PDF]


Home page
jvdiHome page
T. R. Spraker, K. C. VerCauteren, T. Gidlewski, D. A. Schneider, R. Munger, A. Balachandran, and K. I. O'Rourke
Antemortem detection of PrPCWD in preclinical, ranch-raised Rocky Mountain elk (Cervus elaphus nelsoni) by biopsy of the rectal mucosa
J Vet Diagn Invest, January 1, 2009; 21(1): 15 - 24.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
G. Marruchella, C. Ligios, V. Albanese, M. G. Cancedda, L. Madau, G. Lalatta-Costerbosa, M. Mazzoni, P. Clavenzani, R. Chiocchetti, G. Sarli, et al.
Enteroglial and neuronal involvement without apparent neuron loss in ileal enteric nervous system plexuses from scrapie-affected sheep
J. Gen. Virol., October 1, 2007; 88(10): 2899 - 2904.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
A. E. Kincaid and J. C. Bartz
The Nasal Cavity Is a Route for Prion Infection in Hamsters
J. Virol., May 1, 2007; 81(9): 4482 - 4491.
[Abstract] [Full Text] [PDF]


Home page
J. Histochem. Cytochem.Home page
G. Lalatta-Costerbosa, M. Mazzoni, P. Clavenzani, G. Di Guardo, G. Mazzuoli, G. Marruchella, L. De Grossi, U. Agrimi, and R. Chiocchetti
Nitric Oxide Synthase Immunoreactivity and NADPH-d Histochemistry in the Enteric Nervous System of Sarda Breed Sheep With Different PrP Genotypes in Whole-mount and Cryostat Preparations
J. Histochem. Cytochem., April 1, 2007; 55(4): 387 - 401.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
C. Hoffmann, U. Ziegler, A. Buschmann, A. Weber, L. Kupfer, A. Oelschlegel, B. Hammerschmidt, and M. H. Groschup
Prions spread via the autonomic nervous system from the gut to the central nervous system in cattle incubating bovine spongiform encephalopathy
J. Gen. Virol., March 1, 2007; 88(3): 1048 - 1055.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
K. A. Fox, J. E. Jewell, E. S. Williams, and M. W. Miller
Patterns of PrPCWD accumulation during the course of chronic wasting disease infection in orally inoculated mule deer (Odocoileus hemionus).
J. Gen. Virol., November 1, 2006; 87(Pt 11): 3451 - 3461.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
L. Austbo, A. Espenes, I. Olsaker, C. McL. Press, and G. Skretting
Lymphoid follicles of the ileal Peyer's patch of lambs express low levels of PrP, as demonstrated by quantitative real-time RT-PCR on microdissected tissue compartments, in situ hybridization and immunohistochemistry.
J. Gen. Virol., November 1, 2006; 87(Pt 11): 3463 - 3471.
[Abstract] [Full Text] [PDF]


Home page
Vet PatholHome page
E. S. Williams
Chronic Wasting Disease
Vet. Pathol., September 1, 2005; 42(5): 530 - 549.
[Abstract] [Full Text] [PDF]


Home page
Vet PatholHome page
C. Ersdal, M. J. Ulvund, A. Espenes, S. L. Benestad, P. Sarradin, and T. Landsverk
Mapping PrPSc Propagation in Experimental and Natural Scrapie in Sheep with Different PrP Genotypes
Vet. Pathol., May 1, 2005; 42(3): 258 - 274.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
F. Archer, C. Bachelin, O. Andreoletti, N. Besnard, G. Perrot, C. Langevin, A. Le Dur, D. Vilette, A. Baron-Van Evercooren, J.-L. Vilotte, et al.
Cultured Peripheral Neuroglial Cells Are Highly Permissive to Sheep Prion Infection
J. Virol., January 1, 2004; 78(1): 482 - 490.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2003 by the Society for General Microbiology.