HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Beer, C. Articles by Wirth, M. Search for Related Content PubMed PubMed Citation Articles by Beer, C. Articles by Wirth, M. Agricola Articles by Beer, C. Articles by Wirth, M.

Gene expression analysis of murine cells producing amphotropic mouse leukaemia virus at a cultivation temperature of 32 and 37 °C

¹ Molecular Biotechnology, German Research Centre for Biotechnology, GBF, Mascheroder Weg 1, D-38124 Braunschweig, Germany
² Human Genetics, Georg August University, Göttingen, Germany
³ Institute for Experimental Genetics, GSF-National Research Centre for Environment and Health, Neuherberg, Germany

Correspondence
Manfred Wirth
mwi{at}gbf.de

Cultivation of retrovirus packaging cells at 32 °C represents a common procedure to achieve high titres in mouse retrovirus production. Gene expression profiling of mouse NIH 3T3 cells producing amphotropic mouse leukaemia virus 4070A revealed that 10 % of the 1176 cellular genes investigated were regulated by temperature shift (37/32 °C), while 5 % were affected by retrovirus infection. Strikingly, retrovirus production at 32 °C activated the cholesterol biosynthesis/transport pathway and caused an increase in plasma membrane cholesterol levels. Furthermore, these conditions resulted in transcriptional activation of smoothened (smo), patched (ptc) and gli-1; Smo, Ptc and Gli-1, as well as cholesterol, are components of the Sonic hedgehog (Shh) signalling pathway, which directs pattern formation, diversification and tumourigenesis in mammalian cells. These findings suggest a link between cultivation at 32 °C, production of MLV-A and the Shh signalling pathway.

This article has been cited by other articles:

				P. M. O'Callaghan and D. C. James Systems biotechnology of mammalian cell factories Brief Funct Genomic Proteomic, March 7, 2008; (2008) eln012v1. [Abstract] [Full Text] [PDF]