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Molecular characterization of dsRNA segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, W370, of the plant pathogen Rosellinia necatrix

Toru Iwanami^1,

¹ National Institute of Fruit Tree Science, Fujimoto, Tsukuba 305-8605, Japan
² National Institute for Agro-Environmental Sciences, Kan-nondai, Tsukuba 305-8604, Japan

Correspondence
Chuan Zhao Wei
chenwei{at}vesta.ocn.ne.jp

A hypovirulent isolate, W370, of the white root rot fungus Rosellinia necatrix has previously been shown to harbour 12 dsRNA segments. In this study, complete nucleotide sequences of segments 2 and 5 of W370 dsRNAs were determined. The nucleotide sequence of genome segment 2 was 3773 bases long with a single long open reading frame (ORF) encoding 1226 amino acid residues with a predicted molecular mass of approximately 138·5 kDa. The nucleotide sequence of segment 5 was 2089 bases long with a single long ORF, whose deduced polypeptide contained 646 amino acid residues with a predicted molecular mass of about 72 kDa. Comparative analysis showed that the deduced protein sequence of segment 2 had significant homology with the putative VP2 of Colorado tick fever virus (CTFV) and European Eyach virus (EYAV) in the genus Coltivirus, but the deduced protein sequence of segment 5 had no similarity with other virus proteins. Double-shelled spherical particles approximately 80 nm in diameter associated with W370 dsRNAs were observed in a preparation from the mycelial tissue of isolate W370. The results demonstrated that the virus associated with W370 dsRNAs is a novel reovirus of the family Reoviridae. The virus was named Rosellinia anti-rot virus (RArV).

The GenBank accession numbers of the sequences reported in this paper are AB098022 (segment 2) and AB098023 (segment 5).

Present address: National Agricultural Research Center for Kyushu Okinawa Region, Suya 2421, Nishigoshi, Kumamoto 861-1192, Japan.

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