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The most abundantly transcribed human cytomegalovirus gene (2.7) is non-essential for growth in vitro

¹ Section of Infection and Immunity, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XY, UK
² Department of Medical Microbiology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XY, UK

Correspondence
Gavin Wilkinson
WilkinsonGW1{at}cardiff.ac.uk

The most abundantly transcribed HCMV gene (2.7) encodes a 2·7 kb polyadenylated RNA. Although the laboratory-adapted HCMV strains AD169 and Towne possess two copies of the 2.7 gene within an expanded b sequence element, the low passage strain Toledo and all clinical isolates analysed contain only a single copy located within the U_L region. A 2.7 deletion mutant constructed based on a strain Toledo background was shown to replicate with kinetics comparable to those of the parental virus; the 2.7 gene is therefore not essential for virus replication in vitro. Sequencing the 2.7 gene from HCMV clinical isolates and the Toledo strain reveals that although the overall gene sequence is highly conserved (>99 %), the RL4 frame originally assigned in strain AD169 was disrupted in each of these viruses. Consequently, the 2.7 transcript does not encode any obvious translation product and thus may not function as an mRNA.

The GenBank accession numbers of the HCMV sequences reported in this paper are AY325309–AY325312.

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