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Mapping of viral RNA sequences required for assembly of peanut clump virus particles

Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France

Correspondence
Odile Hemmer
odile.hemmer{at}ibmp-ulp.u-strasbg.fr

RNA sequences required for assembly into rod-shaped virions of RNA-1 and RNA-2 of Peanut clump virus (PCV) were mapped by testing the ability of different RNA-1 and -2 deletion mutants to be encapsidated in vivo in an RNase-resistant form. Encapsidation of RNA-1 was found to require a sequence domain in the 5'-proximal part of the P15 gene, the 3'-proximal gene of RNA-1. On the other hand, the subgenomic RNA which encodes P15 was not encapsidated, suggesting that other features of RNA-1 are important as well. Two sequences which could drive encapsidation of RNA-2 deletion mutants were located. One was in the 5'-proximal coat protein gene and the other in the P14 gene near the RNA 3' terminus. There were no obvious sequence homologies between the different assembly initiation sequences.