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J Gen Virol 85 (2004), 173-178; DOI 10.1099/vir.0.19481-0

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© 2004 Society for General Microbiology

Short Communication

Resistance to pseudorabies virus infection in transformed cell lines expressing a soluble form of porcine herpesvirus entry mediator C

Etsuro Ono1, Keiko Amagai1,4, Saori Yoshino1,3, Satoshi Taharaguchi1, Manabu Inobe2 and Toshimitsu Uede2

1 Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan
2 Division of Molecular Immunology, Research Section of Molecular Pathogenesis, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan
3 Gene Techno Science, Sapporo 062-8517, Japan
4 Sankyo Labo Service Corporation, Tokyo 132-0023, Japan

Correspondence
Etsuro Ono
etsuro{at}imm.hokudai.ac.jp

Porcine herpesvirus entry mediator C (HveC) is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine HveC mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) and bovine herpesvirus type 1 (BHV-1). In order to assess the antiviral potential of a soluble form of porcine HveC, Vero cells were transformed with the chimeric gene expressing a fusion protein (PHveCIg) consisting of an extracellular domain of porcine HveC and the Fc portion of human IgG1. The transformed cell lines expressing PHveCIg showed marked resistance to PRV infection. Resistance to infection by other alphaherpesviruses (HSV-1 and BHV-1) was also observed in the transformed cell line. The present results demonstrate that a soluble form of porcine HveC is able to exert a significant antiviral effect against pseudorabies virus and other alphaherpesvirus infection in vitro.




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 Proc. Natl. Acad. Sci. USAHome page
E. Ono, K. Amagai, S. Taharaguchi, Y. Tomioka, S. Yoshino, Y. Watanabe, P. Cherel, L.-M. Houdebine, M. Adam, M. Eloit, et al.
Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock
PNAS, November 16, 2004; 101(46): 16150 - 16155.
[Abstract] [Full Text] [PDF]


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