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J Gen Virol 85 (2004), 179-184; DOI 10.1099/vir.0.19453-0

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© 2004 Society for General Microbiology

Short Communication

Inhibition of human cytomegalovirus replication by small interfering RNAs

Lüder Wiebusch, Matthias Truss and Christian Hagemeier

Department of Pediatrics, Laboratory for Molecular Biology, Charité, CCM-Ziegelstr. 5-9, Humboldt-University, Berlin, Germany

Correspondence
Christian Hagemeier
christian.hagemeier{at}charite.de

Small interfering RNAs (siRNAs) are the mediators of a sequence-specific process of gene silencing called RNA interference (RNAi). Here, we show that synthetic siRNAs against essential gene products of human cytomegalovirus (HCMV) can trigger RNAi in serum-starved, infected primary fibroblasts, as well as in U373 cells, leading to effective inhibition of viral DNA replication. This opens new possibilities for antiviral strategies and for the analysis of viral and cellular genes important to HCMV physiology.




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