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Anticarsia gemmatalis multicapsid nucleopolyhedrovirus v-trex gene encodes a functional 3' to 5' exonuclease

Martin Shapiro

USDA/ARS, Insect Biocontrol Laboratory, BARC-West, Building 011A, Room 214, Beltsville, MD 20852-2350, USA

Correspondence
Jeffrey M. Slack
slackj{at}ba.ars.usda.gov

The viral three-prime repair exonuclease (v-trex) gene of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus (AgMNPV) is the first baculovirus gene to be described with significant homology to a 3' exonuclease. v-trex is an early gene that is expressed by AgMNPV from 3 h post-infection. In the present study, the AgMNPV v-trex ORF was cloned into the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) under the control of a polyhedrin promoter. The resulting virus produced an abundant, soluble protein that migrated with an apparent size of 23·7 kDa. The 3' to 5' exonuclease activity associated with this v-trex-expressing recombinant AcMNPV was 2000-fold above that of wild-type AcMNPV. This exonuclease activity was inhibited by EDTA and was activated in the presence of Mg²⁺ and, to a lesser extent, Mn²⁺. From these results, the AgMNPV v-trex gene is concluded to encode an independently active 3' to 5' exonuclease.

Present address: Department of Entomology, Soils and Plant Sciences, Clemson University, Clemson, SC 29634-0315, USA.

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