HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Patton, G. S. Articles by McClure, M. O. Search for Related Content PubMed PubMed Citation Articles by Patton, G. S. Articles by McClure, M. O. Agricola Articles by Patton, G. S. Articles by McClure, M. O.

Cell-cycle dependence of foamy virus vectors

Otto Erlwein

Jefferiss Research Trust Laboratories, Wright Fleming Institute, Division of Medicine, Imperial College London, London W2 1PG, UK

Correspondence
Myra O. McClure
m.mcclure{at}imperial.ac.uk

Retroviruses differ in the extent to which they are dependent on host-cell proliferation for their replication, an aspect of their replication that impacts on their vector potential. Foamy viruses offer distinct advantages over other retroviruses for development as vectors for gene therapy. A vector derived from the prototypic foamy virus (PFV), formerly known as human foamy virus (HFV), transduced aphidicolin-arrested cells five- to tenfold more efficiently than one derived from murine leukemia virus (MLV), but several-fold less efficiently than a human immunodeficiency virus type 1 (HIV-1) vector. The same relative efficiency was found following transduction of cells that had been arrested by -irradiation or with mitomycin C. Cells that were exposed to vector during aphidicolin arrest and were subsequently allowed to cycle were transduced significantly better by PFV than by MLV. Quiescent human CD34⁺ progenitor cells were transduced as efficiently by PFV as by HIV vectors (40–50 %) when transduction was assayed after the cells were allowed to cycle.

A figure showing the derivation of the constructs expressing the gag and pol genes is available as supplementary material in JGV Online.

Present address: Institute for Biomedical Research, Georg-Speyer-Haus, Paul-Ehrlich-Str. 42–44, D-60596 Frankfurt/Main, Germany.

This article has been cited by other articles:

				L. Jarrosson-Wuilleme, C. Goujon, J. Bernaud, D. Rigal, J.-L. Darlix, and A. Cimarelli Transduction of Nondividing Human Macrophages with Gammaretrovirus-Derived Vectors J. Virol., February 1, 2006; 80(3): 1152 - 1159. [Abstract] [Full Text] [PDF]

				G. S. Patton, S. A. Morris, W. Chung, P. D. Bieniasz, and M. O. McClure Identification of Domains in Gag Important for Prototypic Foamy Virus Egress J. Virol., May 15, 2005; 79(10): 6392 - 6399. [Abstract] [Full Text] [PDF]