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Genetic and phenotypic variations of isolates of shrimp Taura syndrome virus found in Penaeus monodon and Metapenaeus ensis in Taiwan

Yun-Shiang Chang^1,

Shao-En Peng^1,

¹ Institute of Zoology, National Taiwan University, Taipei 106, Taiwan, Republic of China
² Department of Entomology, National Taiwan University, Taipei 106, Taiwan, Republic of China

Correspondence
Chu-Fang Lo
gracelow{at}ntu.edu.tw
Guang-Hsiung Kou
ghkou{at}ccms.ntu.edu.tw

Distinct Taura syndrome virus (TSV) isolates were found in Metapenaeus ensis (isolate Tw2KMeTSV), Penaeus monodon (isolate Tw2KPmTSV) and Litopenaeus vannamei (isolate Tw02LvTSV). Nucleotide sequence analysis of these three isolates revealed differences in the TSV structural protein (capsid protein precursor) gene orf2. TSV ORF2 amino acid sequence comparison and phylogenetic analysis suggested a comparatively close relationship between these three Taiwanese isolates and the Hawaiian isolate HI94TSV. In P. monodon specimens that were naturally and experimentally infected with the Tw2KPmTSV isolate, the virus was contained and shrimps showed no clinical signs of infection. However, when P. monodon was challenged with the Tw2KMeTSV isolate, the virus replicated freely. The ORF2 amino acid sequence of the Tw2KMeTSV isolate differed from that of isolate Tw2KPmTSV in four positions and these differences may account for their phenotypic differences, at least in terms of their ability to replicate in specific hosts.

These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are AY355309–AY355311.

A table showing abbreviations and descriptions of TSV isolates used in this study is available as supplementary material in JGV Online.