| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Agrivirology Laboratory, Research Institute for Bioresources, Okayama University, 2-20-1 Chu-ou, Kurashiki, Okayama 710-0046, Japan
2 Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901-8520, USA
Correspondence
Nobuhiro Suzuki
nsuzuki{at}rib.okayama-u.ac.jp
Bradley I. Hillman
hillman{at}aesop.rutgers.edu
Mycoreovirus 1 (MYRV-1) is the type species of the newly described genus Mycoreovirus of the large virus family Reoviridae. The virus was isolated from a hypovirulent strain (9B21) of the chestnut blight fungus, Cryphonectria parasitica. A previous study showed that double-shelled particles introduced to fungal spheroplasts resulted in stably infected colonies. Of the 11 double-stranded RNA genomic segments (S1–S11), the three largest (S1–S3) were sequenced previously and shown to have moderate levels of similarity to the homologous segments of mammal-pathogenic coltiviruses (Eyach virus and Colorado tick fever virus) and another fungus-infecting reovirus, Mycoreovirus 3 of Rosellinia necatrix strain W370 (MYRV-3/RnW370). The sequences of the remaining segments (S4–S11) are reported here. All of the segments have single ORFs on their positive strands and the terminal sequences 5'-GAUCA----GCAGUCA-3' are conserved among currently and previously sequenced segments. Oligo-cap analysis showed that the positive strands of the genomic segments are capped, whereas the negative strands are not. Similarities among the four evolutionarily related viruses include low or moderate levels of amino acid sequence identity (14·7–34·2 %) and isoelectric points among equivalent polypeptides, e.g. proteins encoded by segments S4 and S5 of the four viruses. Phylogenetic analysis indicated that MYRV-1/Cp9B21 is related more closely to MYRV-3/RnW370 than to the coltiviruses. An interesting dissimilarity is found in codon-choice pattern among the four viruses, i.e. MYRV-1/Cp9B21 segments have a lower frequency of [XYG+XYC] than corresponding segments of the other viruses, suggesting a possible adjustment of virus codon usage to their host environments.
The GenBank/EMBL/DDBJ accession numbers for the sequences described in this paper are AB179636–AB179643.
This article has been cited by other articles:
|
|
 |
|
  M. I. Faruk, A. Eusebio-Cope, and N. Suzuki A Host Factor Involved in Hypovirus Symptom Expression in the Chestnut Blight Fungus, Cryphonectria parasitica J. Virol., January 15, 2008; 82(2): 740 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Deng, T. D. Allen, B. I. Hillman, and D. L. Nuss Comparative Analysis of Alterations in Host Phenotype and Transcript Accumulation following Hypovirus and Mycoreovirus Infections of the Chestnut Blight Fungus Cryphonectria parasitica Eukaryot. Cell, August 1, 2007; 6(8): 1286 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Supyani, B. I. Hillman, and N. Suzuki Baculovirus expression of the 11 mycoreovirus-1 genome segments and identification of the guanylyltransferase-encoding segment J. Gen. Virol., January 1, 2007; 88(1): 342 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sun, D. L. Nuss, and N. Suzuki Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713 J. Gen. Virol., December 1, 2006; 87(12): 3703 - 3714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xie, D. Wei, D. Jiang, Y. Fu, G. Li, S. Ghabrial, and Y. Peng Characterization of debilitation-associated mycovirus infecting the plant-pathogenic fungus Sclerotinia sclerotiorum J. Gen. Virol., January 1, 2006; 87(1): 241 - 249. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
