HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schirrmeier, H. Articles by Beer, M. Search for Related Content PubMed PubMed Citation Articles by Schirrmeier, H. Articles by Beer, M. Agricola Articles by Schirrmeier, H. Articles by Beer, M.

Genetic and antigenic characterization of an atypical pestivirus isolate, a putative member of a novel pestivirus species

Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, Boddenblick 5a, 17493 Greifswald-Insel Riems, Germany

Correspondence
Martin Beer
beer{at}rie.bfav.de

The genus Pestivirus within the family Flaviviridae currently consists of four different main species: Classical swine fever virus, Bovine viral diarrhea virus types 1 and 2 and Border disease virus. A fifth tentative species is represented by an isolate from a giraffe. In this study, a completely new pestivirus, isolated from a batch of fetal calf serum that was collected in Brazil, is described. It is proposed that the isolate D32/00_‘HoBi’ may constitute a novel sixth pestivirus species, because it is genetically, as well as antigenically, markedly different from all other pestiviruses. Based on the entire N^pro- and E2-encoding sequences, identities of <70 % to all other pestivirus species were determined. Similarly, cross-neutralization and binding studies using antisera and mAbs revealed marked antigenic differences between D32/00_‘HoBi’ and all other pestiviruses.

The GenBank/EMBL/DDBJ accession numbers for the 5'-UTR, N^pro, E2, NS3 and 3'-UTR sequences of isolate D32/00_‘HoBi’ reported in this paper are AY489116, AY489117, AY604725, AY713481 and AY604726, respectively.

This article has been cited by other articles:

				T. B. Rasmussen, A. Uttenthal, I. Reimann, J. Nielsen, K. Depner, and M. Beer Virulence, immunogenicity and vaccine properties of a novel chimeric pestivirus J. Gen. Virol., February 1, 2007; 88(2): 481 - 486. [Abstract] [Full Text] [PDF]