| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University, GR-54124 Thessaloniki, Greece
2 Laboratory of Pathology, Faculty of Veterinary Medicine, Aristotle University, GR-54124 Thessaloniki, Greece
3 Laboratory of Epidemiology, Biostatistics and Economics of Animal Production, Faculty of Veterinary Medicine, University of Thessaly, Trikalon 224, GR-43100 Karditsa, Greece
4 Clinic of Productive Animal Medicine, Faculty of Veterinary Medicine, Aristotle University, GR-54124 Thessaloniki, Greece
5 Laboratory of Pharmacology, Department of Pharmaceutical Sciences, Aristotle University, GR-54124 Thessaloniki, Greece
Correspondence
Charalambos Billinis
billinis{at}vet.uth.gr
A total of 216 local crossbred sheep from 16 scrapie-affected Greek flocks and 210 purebred sheep of the milk breeds Chios and Karagouniko from healthy flocks were analysed for scrapie-linked polymorphisms in the prion protein (PrP) gene. Of the 216 sheep in this case–control study, 96 sheep were clinical cases, 25 subclinical cases (asymptomatic at the moment of euthanasia but positive by histopathology and/or ELISA detecting proteinase-resistant PrP) and 95 healthy controls (negative by all evaluations). Polymorphisms at codons 136, 154 and 171 were determined by denaturing gradient gel electrophoresis, followed by RFLP and sequencing. Scrapie, both clinical and subclinical, was associated with the genotypes ARQ/ARQ (88 of 110 sheep of that genotype), ARQ/TRQ (9 of 13), ARQ/AHQ (15 of 38) and VRQ/VRQ (9 of 17). Histopathological lesions were more severe in the clinical cases. Genotypes ARQ/ARR (26 sheep), ARQ/ARK (seven sheep), AHQ/ARR (one sheep), ARH/ARH (one sheep) and ARR/ARH (three sheep) were detected exclusively in healthy control sheep. In the purebred survey, four genotypes were present in the Chios sheep (ARQ/ARQ, ARQ/TRQ, ARQ/AHQ and ARQ/ARR) and four in the Karagouniko sheep (ARQ/ARQ, ARQ/AHQ, ARQ/ARR and ARQ/ARH).
The GenBank accession numbers of the sequences reported in this paper are AJ567981–AJ567988.
Present address: Laboratory of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Trikalon 224, GR-43100 Karditsa, Greece.
This article has been cited by other articles:
|
|
 |
|
  J. T. McKay, T. A. Brigner, B. E. Caplin, K. S. McCurdy, and R. L. Forde A real-time polymerase chain reaction assay to detect single nucleotide polymorphisms at codon 171 in the prion gene for the genotyping of scrapie susceptibility in sheep J Vet Diagn Invest, March 1, 2008; 20(2): 209 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Corbiere, F. Barillet, O. Andreoletti, F. Fidelle, N. Laphitz-Bordet, F. Schelcher, and P. Joly Advanced survival models for risk-factor analysis in scrapie J. Gen. Virol., February 1, 2007; 88(2): 696 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Diaz, Z. G. Vitezica, R. Rupp, O. Andreoletti, and J. M. Elsen Polygenic variation and transmission factors involved in the resistance/susceptibility to scrapie in a Romanov flock J. Gen. Virol., March 1, 2005; 86(3): 849 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Orge, A. Galo, C. Machado, C. Lima, C. Ochoa, J. Silva, M. Ramos, and J. P. Simas Identification of putative atypical scrapie in sheep in Portugal J. Gen. Virol., November 1, 2004; 85(11): 3487 - 3491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Acutis, L. Sbaiz, F. Verburg, M. V. Riina, G. Ru, G. Moda, M. Caramelli, and A. Bossers Low frequency of the scrapie resistance-associated allele and presence of lysine-171 allele of the prion protein gene in Italian Biellese ovine breed J. Gen. Virol., October 1, 2004; 85(10): 3165 - 3172. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
