| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Measles Virus Section, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MS-C22, Atlanta, GA 30333, USA
Correspondence
Paul A. Rota
prota{at}cdc.gov
To study the replication of Nipah virus (NiV), a minigenome replication assay that does not require the use of infectious virus was developed. The minigenome was constructed to encode a NiV vRNA analogue containing the gene for chloramphenicol acetyltransferase (CAT) under the control of putative NiV transcription motifs and flanked by the NiV genomic termini. CAT protein was detected only when plasmids encoding the NiV minigenome, nucleocapsid protein (N), phosphoprotein (P) and polymerase protein (L) were transfected into CV1 cells. To determine whether NiV conforms to the rule of six, a series of plasmids encoding minigenomes that differed in length by a single nucleotide was tested in the replication assay. CAT production was detected only with the minigenome whose length was an even multiple of six. The replication assay was also used to show that the N, P and L proteins of NiV recognize cis-acting sequences in the genomic termini of Hendra virus (HeV) but not measles virus. While these results suggest that NiV uses a replication strategy that is similar to those of other paramyxoviruses, they also support the inclusion of NiV and HeV in a separate genus within the subfamily Paramyxovirinae.
Present address: Australian Animal Health Laboratory, Geelong, Australia.
This article has been cited by other articles:
|
|
 |
|
  M. J. Ciancanelli, V. A. Volchkova, M. L. Shaw, V. E. Volchkov, and C. F. Basler Nipah Virus Sequesters Inactive STAT1 in the Nucleus via a P Gene-Encoded Mechanism J. Virol., August 15, 2009; 83(16): 7828 - 7841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Lo, B. H. Harcourt, B. A. Mungall, A. Tamin, M. E. Peeples, W. J. Bellini, and P. A. Rota Determination of the henipavirus phosphoprotein gene mRNA editing frequencies and detection of the C, V and W proteins of Nipah virus in virus-infected cells J. Gen. Virol., February 1, 2009; 90(2): 398 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Ludlow, M. K. Lo, J. J. Rodriguez, P. A. Rota, and C. M. Horvath Henipavirus V Protein Association with Polo-Like Kinase Reveals Functional Overlap with STAT1 Binding and Interferon Evasion J. Virol., July 1, 2008; 82(13): 6259 - 6271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sleeman, B. Bankamp, K. B. Hummel, M. K. Lo, W. J. Bellini, and P. A. Rota The C, V and W proteins of Nipah virus inhibit minigenome replication J. Gen. Virol., May 1, 2008; 89(5): 1300 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. de Graaf, S. Herfst, E. J. A. Schrauwen, Y. Choi, B. G. van den Hoogen, A. D. M. E. Osterhaus, and R. A. M. Fouchier Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses J. Gen. Virol., April 1, 2008; 89(4): 975 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Walpita and C. J. Peters Cis-acting elements in the antigenomic promoter of Nipah virus J. Gen. Virol., September 1, 2007; 88(9): 2542 - 2551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. White, J.-J. Yoon, J. K. Lee, A. Sun, Y. Du, H. Fu, J. P. Snyder, and R. K. Plemper Nonnucleoside Inhibitor of Measles Virus RNA-Dependent RNA Polymerase Complex Activity Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2293 - 2303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Ciancanelli and C. F. Basler Mutation of YMYL in the Nipah Virus Matrix Protein Abrogates Budding and Alters Subcellular Localization J. Virol., December 15, 2006; 80(24): 12070 - 12078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yoneda, V. Guillaume, F. Ikeda, Y. Sakuma, H. Sato, T. F. Wild, and C. Kai Establishment of a Nipah virus rescue system PNAS, October 31, 2006; 103(44): 16508 - 16513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Govindarajan, U. J. Buchholz, and S. K. Samal Recovery of Avian Metapneumovirus Subgroup C from cDNA: Cross-Recognition of Avian and Human Metapneumovirus Support Proteins. J. Virol., June 1, 2006; 80(12): 5790 - 5797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. N. Pham, S. Biacchesi, M. H. Skiadopoulos, B. R. Murphy, P. L. Collins, and U. J. Buchholz Chimeric Recombinant Human Metapneumoviruses with the Nucleoprotein or Phosphoprotein Open Reading Frame Replaced by That of Avian Metapneumovirus Exhibit Improved Growth In Vitro and Attenuation In Vivo J. Virol., December 15, 2005; 79(24): 15114 - 15122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Weik, S. Enterlein, K. Schlenz, and E. Muhlberger The Ebola Virus Genomic Replication Promoter Is Bipartite and Follows the Rule of Six J. Virol., August 15, 2005; 79(16): 10660 - 10671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. P. Chan, C. L. Koh, S. K. Lam, and L.-F. Wang Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of henipaviruses J. Gen. Virol., June 1, 2004; 85(6): 1675 - 1684. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
