HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Salánki, K. Articles by Balázs, E. Search for Related Content PubMed PubMed Citation Articles by Salánki, K. Articles by Balázs, E. Agricola Articles by Salánki, K. Articles by Balázs, E.

Compatibility of the movement protein and the coat protein of cucumoviruses is required for cell-to-cell movement

¹ Agricultural Biotechnology Center, Szent-Györgyi Albert u. 4, H-2100 Gödöll, Hungary
² Department of Theoretical Chemistry, Eötvös Loránd University, Pázmány Péter Sétány 1/A, H-1117 Budapest, Hungary
³ Protein Modelling Group, Hungarian Academy of Sciences – Eötvös Lóránd University, Pázmány Péter Sétány 1/A, H-1117 Budapest, Hungary

Correspondence
Katalin Salánki
salanki{at}abc.hu

For the cell-to-cell movement of cucumoviruses both the movement protein (MP) and the coat protein (CP) are required. These are not reversibly exchangeable between Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV). The MP of CMV is able to function with the TAV CP (chimera RT), but TAV MP is unable to promote the cell-to-cell movement in the presence of CMV CP (chimera TR). To gain further insight into the non-infectious nature of the TR recombinant, RNA 3 chimeras were constructed with recombinant MPs and CPs. The chimeric MP and one of the CP recombinants were infectious. The other recombinant CP enabled virus movement only after the introduction of two point mutations (GluLys and LysArg at aa 62 and 65, respectively). The mutations served to correct the CP surface electrostatic potential that was altered by the recombination. The infectivity of the TR virus on different test plants was restored by replacing the sequence encoding the C-terminal 29 aa of the MP with the corresponding sequence of the CMV MP gene or by exchanging the sequence encoding the C-terminal 15 aa of the CP with the same region of TAV. The analysis of the recombinant clones suggests a requirement for compatibility between the C-terminal 29 aa of the MP and the C-terminal two-thirds of the CP for cell-to-cell movement of cucumoviruses.

This article has been cited by other articles:

				J. Sztuba-Solinska and J. J. Bujarski Insights into the Single-Cell Reproduction Cycle of Members of the Family Bromoviridae: Lessons from the Use of Protoplast Systems J. Virol., November 1, 2008; 82(21): 10330 - 10340. [Full Text] [PDF]

				L. F. Pacios and F. Garcia-Arenal Comparison of properties of particles of Cucumber mosaic virus and Tomato aspermy virus based on the analysis of molecular surfaces of capsids J. Gen. Virol., July 1, 2006; 87(7): 2073 - 2083. [Abstract] [Full Text] [PDF]

				S. Llamas, I. M. Moreno, and F. Garcia-Arenal Analysis of the viability of coat-protein hybrids between Cucumber mosaic virus and Tomato aspermy virus J. Gen. Virol., July 1, 2006; 87(7): 2085 - 2088. [Abstract] [Full Text] [PDF]