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Evidence of rotavirus intragenic recombination between two sublineages of the same genotype

Karin Bok^3,

¹ Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Río de la Plata y Lagerenza, Asunción (2511), Paraguay
² Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay
³ Laboratorio de Gastroenteritis Virales, Departamento de Virología, INEI-ANLIS ‘Dr Carlos G. Malbrán’, Buenos Aires, Argentina

Correspondence
Gabriel I. Parra
gabriel_parra{at}hotmail.com

Rotavirus G4 prevalence increased during the past decade, with one of the highest prevalences reported during rotavirus surveillance in Argentina. Intragenotype diversity analysis has led to its subdivision into lineages (I and II) and sublineages (Ia–Id). On analysis of Argentine and G4 VP7 sequences from other locations, one Argentine strain (ArgRes1723) appeared to be an intermediate between G4 sublineages Ib and Ic. Similarity and bootscanning analyses and Sawyer's test were carried out to demonstrate the recombinant nature of this strain. It was concluded that intragenic recombination occurred between sequences of sublineages Ib and Ic, with a crossover point between nucleotide positions 336 and 387. This study constitutes the first report of a mechanism of evolution in rotaviruses that is currently considered unusual – a recombination event between two strains of the same rotavirus genotype. These results will help increase current knowledge about rotavirus evolution and divergence, improving our understanding of the adaptation mechanisms used by these viruses.

Present address: Center for Pediatric Research, Children's Hospital of The King's Daughters, Eastern Virginia Medical School, 855 West Brambleton Avenue, Norfolk, VA 23510, USA.

An alignment of the nucleotide sequences of the VP7 gene of the 43 G4 rotaviruses used in the recombination analysis is available in JGV Online.

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